KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
cellobiose | pH 5.0, 30°C | Cerrena unicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Cerrena unicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena unicolor | - |
- |
- |
Cerrena unicolor VKM F-3196 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cerrena unicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
114 | - |
pH 5.0, 30°C | Cerrena unicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | - |
Cerrena unicolor | D-glucose | - |
? | |
cellobiose + H2O | - |
Cerrena unicolor VKM F-3196 | D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, SDS-PAGE | Cerrena unicolor |
Synonyms | Comment | Organism |
---|---|---|
beta-glu | - |
Cerrena unicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Cerrena unicolor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | 25°C: about 85% of maximal activity, 55°C: about 65% of maximal activity | Cerrena unicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
immidiate loss of activity | Cerrena unicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
35 | - |
cellobiose | pH 5.0, 30°C | Cerrena unicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Cerrena unicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7 | pH 3.5: about 35% of maximal activity, pH 7.0: about 50% of maximal activity | Cerrena unicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | the enzyme retains about 60% activity at pH 3-6 and only 20% activity at pH 10 | Cerrena unicolor |
General Information | Comment | Organism |
---|---|---|
metabolism | the interaction of cellobiose dehydrogenase (CDH) and beta-GLU (beta-glucosidase) determines the conversion of cellobiose to cellobionolactone or glucose. The formation of cellobionolactone from cellobiose as a result of the action of CDH depends on the availability of quinones (for CDH), which are formed by the interaction of laccase with a phenolic compound. At low quinone concentrations, cellobiose is transformed by beta-GLU to glucose | Cerrena unicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
74.4 | - |
cellobiose | pH 5.0, 30°C | Cerrena unicolor |