Literature summary for 3.2.1.21 extracted from

Fourage, L.; Colas, B.
Synthesis of beta-D-glucosyl- and beta-D-fucosyl-glucoses using beta-glycosidase from Thermus thermophilus (2001), Appl. Microbiol. Biotechnol., 56, 406-410 .

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Application

Application	Comment	Organism
synthesis	the enzyme can constitute a valuable biocatalyst for the synthesis of disaccharides involving beta(1-3) linked disaccharide structures as, for example, Bifidus factors	Thermus thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed in Escherichia coli	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme shows transglycosylase activities, the enzyme catalyze the transfer of beta-D-glucosyl and beta-D-fucosyl from the corresponding 4-nitrophenyl-beta-D-glycopyranosides to a hydroxyl group of glucose in the synthesis of beta-D-glucosyl-D-glucopyranoses and beta-Dfucosyl-D-glucopyranoses. The yields in disaccharides produced under conditions of non-initial velocity are very attractive and the formation of the beta(1-3) linked disaccharides is largely favored	Thermus thermophilus	?	-	?

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Release 2023.1 (January 2023)