Crystallization (Comment) | Organism |
---|---|
in ligand-free form, and in complex form with D-glucose, with inhibitor isofagomine, and D-fucose, wild-type and mutants in residue N223, to 1.2-1.9 A resolution | uncultured microorganism |
Protein Variants | Comment | Organism |
---|---|---|
N223Q | mutation results in reduced glucose tolerance and transglycosylation activity, and drastically changed substrate specificity | uncultured microorganism |
N223Y | mutation results in reduced glucose tolerance and transglycosylation activity, and drastically changed substrate specificity | uncultured microorganism |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
4-nitrophenyl beta-D-fucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
0.21 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
0.39 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
0.71 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
0.85 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
2.65 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
3.61 | - |
4-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
3.99 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
5 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
7.69 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
17.6 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
34.3 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured microorganism | A0A1E1FFN6 | isolated from a compost metagenome | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
uncultured microorganism | 4-nitrophenol + D-fucose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
uncultured microorganism | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
uncultured microorganism | 4-nitrophenol + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Td2F2 | - |
uncultured microorganism |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
assay at | uncultured microorganism |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.94 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
16 | - |
4-nitrophenyl beta-D-fucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
17.6 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
30.8 | - |
4-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
32.3 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
36.7 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
38.7 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
42.5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
69 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
80.4 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
82.8 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
145 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
assay at | uncultured microorganism |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.83 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
2.41 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
5.53 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
8.52 | - |
4-nitrophenyl beta-D-galactopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
16.1 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Q, pH 5.5, 75°C | uncultured microorganism | |
24.7 | - |
4-nitrophenyl beta-D-galactopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
26.1 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
32.3 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism | |
36.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C | uncultured microorganism | |
43.2 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
98.3 | - |
4-nitrophenyl beta-D-fucopyranoside | mutant N223Y, pH 5.5, 75°C | uncultured microorganism | |
115 | - |
4-nitrophenyl beta-D-fucopyranoside | wild-type, pH 5.5, 75°C | uncultured microorganism |