Literature summary for 3.2.1.21 extracted from

Toyama, D.; de Morais, M.; Ramos, F.; Zanphorlin, L.; Tonoli, C.; Balula, A.; de Miranda, F.; Almeida, V.; Marana, S.; Ruller, R.; Murakami, M.; Henrique-Silva, F.
A novel beta-glucosidase isolated from the microbial metagenome of Lake Poraque (Amazon, Brazil) (2018), Biochim. Biophys. Acta, 1866, 569-579 .

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	unidentified microorganism

Crystallization (Commentary)

Crystallization (Comment)	Organism
to 2.75 A resolution. The predicted aglycone region is surrounded by hydrophobic residues Val220, Pro294, and Ala237. The residues His174 and Trp342 delimit the access to the -1 subsite and are critical to define the active site architecture	unidentified microorganism

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	dimer structure, SAXS scattering and energetic analysis	unidentified microorganism

Organism

Organism	UniProt	Comment	Textmining
unidentified microorganism	A0A2I2LGB3	microbial metagenome of Lake Poraque, Amazon, Brazil	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O	-	unidentified microorganism	4-nitrophenol + beta-D-galactose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	unidentified microorganism	4-nitrophenol + beta-D-glucose	-	?
cellobiose + H2O	-	unidentified microorganism	2 D-glucose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 52900, calculated	unidentified microorganism

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	unidentified microorganism

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
35	-	96% of maximum activity	unidentified microorganism

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	30 min, 60% residual activity	unidentified microorganism
46.7	-	melting temperature	unidentified microorganism

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	unidentified microorganism

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	6.5	-	unidentified microorganism

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Release 2023.1 (January 2023)