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Literature summary for 3.2.1.21 extracted from

  • Karnaouri, A.; Topakas, E.; Paschos, T.; Taouki, I.; Christakopoulos, P.
    Cloning, expression and characterization of an ethanol tolerant GH3 beta-glucosidase from Myceliophthora thermophila (2013), PeerJ, 1, e46 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM, 85% residual activity Thermothelomyces thermophilus
EDTA 10 mM, 90% residual activity Thermothelomyces thermophilus
SDS 10 mM, 91% residual activity Thermothelomyces thermophilus
Urea 10 mM, 95% residual activity Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Thermothelomyces thermophilus
2.64
-
 cellobiose pH 5.0, 50°C Thermothelomyces thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothelomyces thermophilus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 10 mM, 208% of initial activity Thermothelomyces thermophilus
Mn2+ 10 mM, 167% of initial activity Thermothelomyces thermophilus
Zn2+ 10 mM, 149% of initial activity Thermothelomyces thermophilus

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QDN2
-
-
Thermothelomyces thermophilus ATCC 42464 G2QDN2
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.6
-
 substrate lichenan. It , pH 5.0, 50°C Thermothelomyces thermophilus
30.7
-
 substrate cellobiose, pH 5.0, 50°C Thermothelomyces thermophilus
52
-
 substrate laminarin, pH 5.0, 50°C Thermothelomyces thermophilus
97.7
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 50°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermothelomyces thermophilus ATCC 42464 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Thermothelomyces thermophilus 2 D-glucose
-
 ?
cellobiose + H2O
-
 Thermothelomyces thermophilus ATCC 42464 2 D-glucose
-
 ?
laminarin + H2O
-
 Thermothelomyces thermophilus laminarioligosaccharides + D-glucose
-
 ?
laminarin + H2O
-
 Thermothelomyces thermophilus ATCC 42464 laminarioligosaccharides + D-glucose
-
 ?
lichenan + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
lichenan + H2O
-
 Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
additional information enzyme hydrolyzes low molecular weight substrates and polysaccharides containing beta-glucosidic residues, and efficiently synthesizes methyl-D-glucoside in the presence of methanol due to its transglycosylation activity. It shows no detectable activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, carboxymethyl cellulose, wheat and birchwood xylans and avicel Thermothelomyces thermophilus ?
-
 ?
additional information enzyme hydrolyzes low molecular weight substrates and polysaccharides containing beta-glucosidic residues, and efficiently synthesizes methyl-D-glucoside in the presence of methanol due to its transglycosylation activity. It shows no detectable activity towards 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, carboxymethyl cellulose, wheat and birchwood xylans and avicel Thermothelomyces thermophilus ATCC 42464 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE, x * 79819, calculated Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
bgl3a
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 143 min Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
6
-
 more than 80% of maximum activity Thermothelomyces thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 24 h, almost 90% residual activity Thermothelomyces thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces thermophilus calculated
-
 5.1