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Literature summary for 3.2.1.21 extracted from

  • Ximenes, E.; Felix, C.; Ulhoa, C.
    Production of cellulases by Aspergillus fumigatus and characterization of one beta-glucosidase (1996), Curr. Microbiol., 32, 119-123 .
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2 mM, 80% residual activity Aspergillus fumigatus
Co2+ 2 mM, 72% residual activity Aspergillus fumigatus
D-glucono-1,5-lactone 2 mM, 60% residual activity Aspergillus fumigatus
D-glucose competitive Aspergillus fumigatus
Hg2+ 2 mM, 54% residual activity Aspergillus fumigatus
K+ 2 mM, 69% residual activity Aspergillus fumigatus
Na+ 2 mM, 68% residual activity Aspergillus fumigatus
SDS 2 mM, 76% residual activity Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
 cellobiose pH 5.0, 42°C Aspergillus fumigatus
1.36
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 42°C Aspergillus fumigatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 PAGE Aspergillus fumigatus

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2 2.5 pH 5.0, 42°C Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus fumigatus 4-nitrophenol + D-glucose
-
 ?
6-bromonaphthyl beta-D-glucopyranoside + H2O
-
 Aspergillus fumigatus 6-bromonaphthol + D-glucose
-
 ?
cellobiose + H2O
-
 Aspergillus fumigatus 2 D-glucose
-
 ?
additional information no substrates: beta-glucans, laminarin, and 4-nitrophenyl-beta-D-xilopyranoside Aspergillus fumigatus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 90000 plus 1 * 45000, SDS-PAGE Aspergillus fumigatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate cellobiose Aspergillus fumigatus
65
-
 substrate 4-nitrophenyl beta-D-glucopyranoside Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 1.5 h, 100% residual activtiy, 3 h, 60% residual activity Aspergillus fumigatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 substrate cellobiose Aspergillus fumigatus
5
-
 substrate 4-nitrophenyl beta-D-glucopyranoside Aspergillus fumigatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5
-
 D-glucose pH 5.0, 42°C Aspergillus fumigatus

Expression

Organism Comment Expression
Aspergillus fumigatus expression is induced by growth on filter paper up