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Literature summary for 3.2.1.21 extracted from
- High-level production of a thermoacidophilic beta-glucosidase from Penicillium citrinum YS40-5 by solid-state fermentation with rice bran (2010), Biores. Technol., 101, 1310-1317 .
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | a 3.43fold synergistic effect by combining with Trichoderma reesei cellulases is observed | Penicillium citrinum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 10 mM, 33% residual activity | Penicillium citrinum |  |
| D-glucose | product inhibition, competitive | Penicillium citrinum | |
| additional information | not inhibitory: EDTA, dithiothreitol | Penicillium citrinum | |
| Zn2+ | 10 mM, 78% residual activity | Penicillium citrinum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 55°C | Penicillium citrinum | |
| 32.2 | - |
cellobiose | pH 5.0, 55°C | Penicillium citrinum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium citrinum | EU918770 | - |
- |
| Penicillium citrinum YS40-5 | EU918770 | - |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 22°C, 4 weeks, more than 95% residual activity | Penicillium citrinum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Penicillium citrinum | 4-nitrophenol + D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Penicillium citrinum YS40-5 | 4-nitrophenol + D-glucose | - |
? | |
| cellobiose + H2O | - |
Penicillium citrinum | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Penicillium citrinum YS40-5 | 2 D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Penicillium citrinum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 75 | more than 80% of maximum activity | Penicillium citrinum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 58 | - |
half-life 2 h | Penicillium citrinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Penicillium citrinum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 10 | - |
Penicillium citrinum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.3 | - |
D-glucose | substrate cellobiose, pH 5.0, 55°C | Penicillium citrinum | |
| 17.6 | - |
D-glucose | substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 55°C | Penicillium citrinum | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Penicillium citrinum | rice bran-based solid-state fermentation of Penicillium citrinum YS40-5 leads to a high yield of beta-glucosidase | additional information |
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