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Literature summary for 3.2.1.21 extracted from

  • Gudmundsson, M.; Hansson, H.; Karkehabadi, S.; Larsson, A.; Stals, I.; Kim, S.; Sunux, S.; Fujdala, M.; Larenas, E.; Kaper, T.; Sandgren, M.
    Structural and functional studies of the glycoside hydrolase family 3 beta-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii (2016), Acta Crystallogr. Sect. D, 72, 860-870 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Application

Application Comment Organism
degradation enzyme shows increased thermal stability and saccharification yield on pretreated corn stover compared with Hypocrea jecorina Cel3A Rasamsonia emersonii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Hypocrea jecorina Rasamsonia emersonii

Crystallization (Commentary)

Crystallization (Comment) Organism
to 0.98 A resolution. Enzyme is a highly N-glycosylated three-domain dimeric protein Rasamsonia emersonii
to 2.2 A resolution. Enzyme is a highly N-glycosylated three-domain dimeric protein Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
0.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
0.4
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
0.4
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
0.78
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii Q8TGI8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein protein is highly N-glycosylated Rasamsonia emersonii
glycoprotein crystallization data Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 2-chloro-4-nitrophenol + D-glucose
-
 ?
2-chloro-4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 2-chloro-4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Rasamsonia emersonii 2 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Rasamsonia emersonii 3 D-glucose
-
 ?
additional information enzyme shows a preference for disaccharide substrates over longer glucooligosaccharides Rasamsonia emersonii ?
-
 ?

Subunits

Subunits Comment Organism
dimer crystallization data Rasamsonia emersonii
dimer gel filtration and crystallization data Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
Cel3A
-
 Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
87.3
-
 melting temperature Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.72
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
5.4
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
5.5
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii
14
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
14
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
55
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.9
-
 cellotriose pH 5.0, 37°C Rasamsonia emersonii
7.1
-
 cellobiose pH 5.0, 37°C Rasamsonia emersonii
14
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
36
-
 2-chloro-4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii
36
-
 2-chloro-4-nitrophenyl beta-D-glucopyranoside pH 5.0, 37°C Rasamsonia emersonii