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Literature summary for 3.2.1.21 extracted from

  • Zeuner, B.; Nyffenegger, C.; Mikkelsen, J.D.; Meyer, A.S.
    Thermostable beta-galactosidases for the synthesis of human milk oligosaccharides (2016), N. Biotechnol., 33, 355-60.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus
expression in Escherichia coli Niallia circulans

Organism

Organism UniProt Comment Textmining
Niallia circulans E5RWQ2
-
-
Thermus thermophilus Q9RA61
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + N,N'-diacetylchitobiose
-
 Thermus thermophilus Gal-beta-(1->4)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
-
 ?
lactose + N,N'-diacetylchitobiose
-
 Niallia circulans Gal-beta-(1->4)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc + H2O
-
 ?
additional information enzyme is able to catalyze formation of lacto-N-neotetraose, i.e. Gal-beta(1,4)-GlcNAc-beta(1,3)-Gal-beta(1,4)-Glc, from lactose and N,N'-diacetylchitobiose and of N-acetyllactosamine, i.e Gal-beta(1,4)-GlcNAc from N-acetylglucosamine and lactose. When compared with truncated beta-galactosidase BgaD-D from Bacillus circulans, and beta-glycosidase CelB from Pyrococcus furiosus, BgaD is the most potent transgalactosidase, but both BglT and CelB can catalyze formation of LNnT and LacNAc, with BglT giving higher yields than CelB Thermus thermophilus ?
-
 ?
additional information enzyme is able to catalyze formation of lacto-N-neotetraose, i.e. Gal-beta-(1->4)-GlcNAc-beta-(1->3)-Gal-beta (1->4)-Glc, from lactose and N,N'-diacetylchitobiose and of N-acetyllactosamine, i.e Gal-beta-(1->4)-GlcNAc from N-acetylglucosamine and lactose. When compared with beta-glycosidase BglT from Thermus thermophilus, and beta-glycosidase CelB from Pyrococcus furiosus, BgaD is the most potent transgalactosidase, but both BglT and CelB can catalyze formation of LNnT and LacNAc, with BglT giving higher yields than CelB Niallia circulans ?
-
 ?
N-acetyl-D-glucosamine + lactose
-
 Niallia circulans 4-O-beta-D-galactopyranosyl-N-acetyl-D-glucosamine + D-glucose transgalactosylation reaction ?
N-acetylglucosamine + lactose
-
 Thermus thermophilus Gal-beta-(1->4)-GlcNAc + D-glucose transgalactosylation reaction ?

Synonyms

Synonyms Comment Organism
BGA
-
 Niallia circulans
BglT
-
 Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half-life 280 min Thermus thermophilus
80
-
 half-life 280 min Niallia circulans
90
-
 half-life 205 min Thermus thermophilus
90
-
 half-life 205 min Niallia circulans
95
-
 half-life 11 min Thermus thermophilus
95
-
 half-life 11 min Niallia circulans