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Literature summary for 3.2.1.21 extracted from

  • Haq, I.U.; Khan, M.A.; Muneer, B.; Hussain, Z.; Afzal, S.; Majeed, S.; Rashid, N.; Javed, M.M.; Ahmad, I.
    Cloning, characterization and molecular docking of a highly thermostable beta-1,4-glucosidase from Thermotoga petrophila (2012), Biotechnol. Lett., 34, 1703-1709.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
gene bglA, recombinant expression in Escherichia coli strain BL21 Codon-Plus Thermotoga petrophila

Inhibitors

Inhibitors Comment Organism Structure
1-propanol 40% v/v, almost complete inhibition Thermotoga petrophila
Cu2+ 20% inhibition at 1 mM Thermotoga petrophila
ethanol 40% v/v, almost complete inhibition Thermotoga petrophila
Hg2+ 90% inhibition at 1 mM Thermotoga petrophila
methanol 40% v/v, almost complete inhibition Thermotoga petrophila
additional information the enzyme is not inhibited by glucose up to 5% w/v Thermotoga petrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila
4.3
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
25
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51500
-
 x * 51500 SDS-PAGE Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila A5IL97 gene bglA
-
Thermotoga petrophila RKU-1 A5IL97 gene bglA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21 Codon-Plus Thermotoga petrophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 purified recombinant enzyme, substrate cellobiose, pH 7.0, 90°C Thermotoga petrophila
1520
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 7.0, 90°C Thermotoga petrophila
9400
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-cellobioside, pH 7.0, 90°C Thermotoga petrophila
14000
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-galactopyranoside, pH 7.0, 90°C Thermotoga petrophila
29200
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-fucopyranoside, pH 7.0, 90°C Thermotoga petrophila
30400
-
 purified recombinant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 7.0, 90°C Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila RKU-1 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-xylopyranose
-
 ?
cellobiose + H2O
-
 Thermotoga petrophila 2 beta-D-glucose
-
 ?
cellobiose + H2O
-
 Thermotoga petrophila RKU-1 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 51500 SDS-PAGE Thermotoga petrophila

Synonyms

Synonyms Comment Organism
BglA
-
 Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80 90
-
 Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 activity range, profile overview Thermotoga petrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5300
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila
17500
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
87400
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Thermotoga petrophila

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, profile overview Thermotoga petrophila

General Information

General Information Comment Organism
additional information enzyme-substrate docking studies, overview Thermotoga petrophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
210
-
 4-nitrophenyl-beta-D-xylopyranoside pH 7.0, 90°C Thermotoga petrophila
4100
-
 4-nitrophenyl-beta-D-cellobioside pH 7.0, 90°C Thermotoga petrophila
30800
-
 4-nitrophenyl-beta-D-glucopyranoside pH 7.0, 90°C Thermotoga petrophila