Any feedback?
Literature summary for 3.2.1.21 extracted from
- A novel beta-glucosidase from Humicola insolens with high potential for untreated waste paper conversion to sugars (2014), Appl. Biochem. Biotechnol., 173, 391-408.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | complete inhibition at 1 mM | Humicola insolens |  |
| Cu2+ | 52% inhibition at 1 mM, 89% at 5 mM | Humicola insolens | |
| D-glucose | competitive inhibition | Humicola insolens | |
| D-mannose | competitive inhibition | Humicola insolens | |
| D-xylose | competitive inhibition | Humicola insolens | |
| Hg2+ | 33% inhibition at 1 mM, 48% at 5 mM | Humicola insolens | |
| additional information | no inhibition by EDTA and insensitive to fructose, ribose, galactose, and L-arabinose up to 50 mM | Humicola insolens | |
| Pb2+ | 45% inhibition at 1 mM, 61% at 5 mM | Humicola insolens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.081 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens | |
| 0.081 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
| 0.24 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens | |
| 0.65 | - |
Salicin | pH 5.0, 50°C | Humicola insolens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on enzyme activity by Ca2+, Sr2+, Co2+, Zn2+, Ni2+, Mg2+, and Mn2+ at up to 5 mM and by Fe2+ and Cr3+ at 1 mM, no metalloenzyme | Humicola insolens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 116000 | - |
4 * 116000, SDS-PAGE | Humicola insolens |
| 404000 | - |
gel filtration | Humicola insolens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | Humicola insolens | - |
2 beta-D-glucose | - |
? | |
| cellobiose + H2O | Humicola insolens RP86 | - |
2 beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Humicola insolens | - |
from feces of Lama glama at the Ribeirao Preto Municipal Zoo (SP, Brazil) | - |
| Humicola insolens RP86 | - |
from feces of Lama glama at the Ribeirao Preto Municipal Zoo (SP, Brazil) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 23.6fold from crude culture extract by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, ultrafiltration, again gel filtration, followed by hyrophobic interaction chromatography and ultrafiltration | Humicola insolens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | solid state fermentation | Humicola insolens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45.6 | - |
purified enzyme, pH 5.0, 50°C, substrate cellobiose | Humicola insolens |
| 46.2 | - |
purified enzyme, pH 5.0, 50°C, substrate 4-nitrophenyl-beta-D-glucopyranoside | Humicola insolens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Humicola insolens RP86 | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Humicola insolens | 4-nitrophenol + D-xylose | - |
? | |
| 4-nitrophenyl beta-D-xyloside + H2O | - |
Humicola insolens RP86 | 4-nitrophenol + D-xylose | - |
? | |
| cellobiose + H2O | - |
Humicola insolens | 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | - |
Humicola insolens RP86 | 2 beta-D-glucose | - |
? | |
| additional information | no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-furanoside, lactose, maltose, trehalose, and sucrose, and poor activity with carboxymethyl cellulose, and Avicel, substrate specificity, overview | Humicola insolens | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-furanoside, lactose, maltose, trehalose, and sucrose, and poor activity with carboxymethyl cellulose, and Avicel, substrate specificity, overview | Humicola insolens RP86 | ? | - |
? | |
| salicin + H2O | - |
Humicola insolens | ? | - |
? | |
| salicin + H2O | - |
Humicola insolens RP86 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 116000, SDS-PAGE | Humicola insolens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BglHi2 | - |
Humicola insolens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Humicola insolens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 75 | activity range, profile overview | Humicola insolens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified enzyme, pH 5.0, 60 min, stable at | Humicola insolens |
| 55 | - |
purified enzyme, pH 5.0, 60 min, 71% activity remaining | Humicola insolens |
| 60 | - |
purified enzyme, pH 5.0, half-life is 47.3 min | Humicola insolens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.2 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
| 343.5 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens | |
| 404.8 | - |
Salicin | pH 5.0, 50°C | Humicola insolens | |
| 3132 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Humicola insolens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8 | activity range, 75% of maximal activity at pH 4.5 and pH 7.5, profile overview | Humicola insolens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.27 | - |
D-glucose | pH 5.0, 50°C | Humicola insolens | |
| 23.7 | - |
D-mannose | pH 5.0, 50°C | Humicola insolens | |
| 42.7 | - |
D-xylose | pH 5.0, 50°C | Humicola insolens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Humicola insolens | isoelectric focusing | - |
5.6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | synergism of the purified enzyme BglHi2 and Trichderma reesei cellulase in the hydrolysis reaction, overview | Humicola insolens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.16 | - |
4-nitrophenyl-beta-D-xyloside | pH 5.0, 50°C | Humicola insolens | |
| 622.7 | - |
Salicin | pH 5.0, 50°C | Humicola insolens | |
| 1305 | - |
cellobiose | pH 5.0, 50°C | Humicola insolens | |
| 4241 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 50°C | Humicola insolens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
