Literature summary for 3.2.1.21 extracted from

Ferrara, M.C.; Cobucci-Ponzano, B.; Carpentieri, A.; Henrissat, B.; Rossi, M.; Amoresano, A.; Moracci, M.
The identification and molecular characterization of the first archaeal bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase demonstrate that family GH116 is made of three functionally distinct subfamilies (2014), Biochim. Biophys. Acta, 1840, 367-377.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Saccharolobus solfataricus

Inhibitors

Inhibitors	Comment	Organism	Structure
(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol	-	Saccharolobus solfataricus
conduritol beta-epoxide	-	Saccharolobus solfataricus
N-butyldeoxynojirimycin	-	Saccharolobus solfataricus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.8	-	4-nitrophenyl beta-D-glucoside	pH 4.0, 60°C	Saccharolobus solfataricus
0.9	-	4-methylumbelliferyl beta-D-glucopyranoside	pH 4.0, 60°C	Saccharolobus solfataricus
2.1	-	cellobiose	pH 4.0, 60°C	Saccharolobus solfataricus
10.8	-	N,N'-diacetyl-beta-chitobiose	pH 4.0, 60°C	Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
93400	-	3 * 93400, SDS-PAGE	Saccharolobus solfataricus
93418	-	3 * 93418, calculated from sequence	Saccharolobus solfataricus
271000	-	gel filtration	Saccharolobus solfataricus
313000	-	gel filtration	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97UH8	-	-
Saccharolobus solfataricus P2	Q97UH8	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.5	-	pH 4.0, 60°C	Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-nitrophenyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus	2-nitrophenol + beta-D-glucose	-	?
2-nitrophenyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus P2	2-nitrophenol + beta-D-glucose	-	?
2-nitrophenyl cellobioside + H2O	-	Saccharolobus solfataricus	beta-D-glucose + ?	-	?
2-nitrophenyl cellobioside + H2O	-	Saccharolobus solfataricus P2	beta-D-glucose + ?	-	?
4-methylumbelliferyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus	4-methylumbelliferol + beta-D-glucose	-	?
4-methylumbelliferyl beta-D-glucopyranoside + H2O	-	Saccharolobus solfataricus P2	4-methylumbelliferol + beta-D-glucose	-	?
4-nitrophenyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus	4-nitrophenol + beta-D-glucose	-	?
4-nitrophenyl beta-D-glucoside + H2O	-	Saccharolobus solfataricus P2	4-nitrophenol + beta-D-glucose	-	?
4-nitrophenyl N,N'-diacetyl-beta-chitobioside + H2O	-	Saccharolobus solfataricus	?	-	?
cellobiose + H2O	-	Saccharolobus solfataricus	2 beta-D-glucose	-	?
cellobiose + H2O	-	Saccharolobus solfataricus P2	2 beta-D-glucose	-	?
cellopentaose + H2O	-	Saccharolobus solfataricus	beta-D-glucose + ?	-	?
cellotetraose + H2O	-	Saccharolobus solfataricus	beta-D-glucose + ?	-	?
N,N'-diacetyl-beta-chitobiose + H2O	-	Saccharolobus solfataricus	N-acetyl-D-glucosamine	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 93400, SDS-PAGE	Saccharolobus solfataricus
trimer	3 * 93418, calculated from sequence	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
bifunctional exo-beta-glucosidase/N-acetyl-beta-glucosaminidase	-	Saccharolobus solfataricus
SSO3039	-	Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Saccharolobus solfataricus
70	-	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
65	-	stable for 24 h, about 20% loss of activity after 48 h	Saccharolobus solfataricus
85	-	about 45% loss of activity after 8 h, about 60% loss of activity after 24 h	Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.56	-	N,N'-diacetyl-beta-chitobiose	pH 4.0, 60°C	Saccharolobus solfataricus
1.8	-	cellobiose	pH 4.0, 60°C	Saccharolobus solfataricus
8.8	-	4-methylumbelliferyl beta-D-glucopyranoside	pH 4.0, 60°C	Saccharolobus solfataricus
9.15	-	4-nitrophenyl beta-D-glucoside	pH 4.0, 60°C	Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	-	Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00515	-	(2R,3R,4R,5S)-1-butyl-2-(hydroxymethyl)piperidine-3,4,5-triol	pH 4.0, 60°C	Saccharolobus solfataricus
9	-	conduritol beta-epoxide	pH 4.0, 60°C	Saccharolobus solfataricus

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.00515	-	pH 7.0, 45°C	Saccharolobus solfataricus	N-butyldeoxynojirimycin
9	-	pH 7.0, 45°C	Saccharolobus solfataricus	conduritol beta-epoxide

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.05	-	N,N'-diacetyl-beta-chitobiose	pH 4.0, 60°C	Saccharolobus solfataricus
0.9	-	cellobiose	pH 4.0, 60°C	Saccharolobus solfataricus
9.9	-	4-methylumbelliferyl beta-D-glucopyranoside	pH 4.0, 60°C	Saccharolobus solfataricus
11.4	-	4-nitrophenyl beta-D-glucoside	pH 4.0, 60°C	Saccharolobus solfataricus

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Release 2023.1 (January 2023)