Literature summary for 3.2.1.21 extracted from

Hansson, T.; Adlercreutz, P.
Enhanced transglucosylation/hydrolysis ratio of mutants of Pyrococcus furiosus beta-glucosidase: effects of donor concentration, water content, and temperature on activity and selectivity in hexanol (2001), Biotechnol. Bioeng., 75, 656-665.

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Organism

Organism	UniProt	Comment	Textmining

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.03	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 95°C, mutant enzyme M424K/F426Y	Pyrococcus furiosus
0.04	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 95°C, mutant enzyme F426Y	Pyrococcus furiosus
0.13	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 95°C, mutant enzyme M424K	Pyrococcus furiosus
0.14	-	4-nitrophenyl beta-D-glucoside	pH 5.0, 95°C, wild-type enzyme	Pyrococcus furiosus

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Release 2023.1 (January 2023)