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Literature summary for 3.2.1.21 extracted from
- Beta-glucosidase isolated from soybean okara shows specificity toward glucosyl isoflavones (2010), J. Agric. Food Chem., 58, 8872-8878.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glucose | - |
Glycine max |  |
| glucuronic acid | - |
Glycine max | |
| mannose | - |
Glycine max | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | inhibits activity | Glycine max | |
| Hg2+ | inhibits activity | Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
SDS-PAGE | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using CM-sepharose chromatography | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme possesses strict specificity toward glucosyl isoflavones but not malonylglucosidic conjugates of isoflavones of soybean | Glycine max | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1* 75000, SDS-PAGE | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | under pH 4.5, the relative activity of beta-glucosidase at 30°C and 60°C is about 44% and 40%. After incubation at 70°C and pH 4.5 for 1 h, beta-glucosidase still retains about 85% of its original activity | Glycine max |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Glycine max |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | about 80% of the relative activity at 45°C is retained at both pH 4.0 and 5.0. 5% activity at pH 3.5 | Glycine max |
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Release 2023.1 (January 2023)
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