KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
tuberonic acid beta-D-glucoside | pH 4.0, 37°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
1 * 42000 + 1 * 26000, SDS-PAGE | Oryza sativa |
42000 | - |
1 * 42000 + 1 * 26000, SDS-PAGE | Oryza sativa |
65000 | - |
gel filtration | Oryza sativa |
66000 | - |
native-PAGE | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Oryza sativa |
Purification (Comment) | Organism |
---|---|
ca. 4300fold, yield of 1.1%, ammonium sulfate precipitation, CM-Sepharose, ConA Sepharose, Superdex 200, Resource S, hydroxyapatite | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
inflorescence | during grain-filling | Oryza sativa | - |
leaf | - |
Oryza sativa | - |
seed | purification of OsTAGG1 from immature rice seeds | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.73 | - |
tuberonic acid beta-D-glucoside as substrate, 4300fold purified protein, pH 4.0, 37°C | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-O-beta-D-glucopyranosylsalicylate + H2O | 25% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside | Oryza sativa | salicylic acid + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucoside + H2O | 178% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside | Oryza sativa | 4-nitrophenol + beta-D-glucose | - |
? | |
methyl tuberonic acid beta-D-glucoside + H2O | i.e. 12-hydroxyjasmonic acid beta-d-glucosid methyl ester, 80% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside | Oryza sativa | methyl tuberonic acid + beta-D-glucose | - |
? | |
additional information | jasmonic acid beta-D-glucosyl ester + H2O, product: jasmonic acid + beta-D-glucose, 51% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside | Oryza sativa | ? | - |
? | |
additional information | no or negligible hydrolyzing activity with zeatin-O-glucoside, linamarin, or 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-galactoside, 4-nitrophenyl-beta-D-cellobioside, and 4-nitrophenyl-alpha-D-glucoside | Oryza sativa | ? | - |
? | |
tuberonic acid beta-D-glucoside + H2O | - |
Oryza sativa | tuberonic acid + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 42000 + 1 * 26000, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
OsTAGG | OsTAGG1 and OsTAGG2, detected by CM-Sepharose chromatography, at least two isoforms in rice panicles | Oryza sativa |
tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase | a specific beta-glucosidase hydrolyzing tuberonic acid beta-D-glucose | Oryza sativa |
tuberonic acid glucoside beta-glucosidase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
after 15 min at various temperatures ranging from 30 to 70°C, the original activity retained up to 50°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Oryza sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.3 | 10.3 | fairly stable, 90% of the original activity retains after incubation at 4°C for 16 h | Oryza sativa |