Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 40 mmol/l, 233.33% relative enzyme activity in midgut, 193.31% relative enzyme activity in salivary glands | Chilo suppressalis | |
MgCl2 | 40 mmol/l, 200% relative enzyme activity in midgut, 169.49% relative enzyme activity in salivary glands | Chilo suppressalis | |
NaCl | 40 mmol/l, 198.57% relative enzyme activity in midgut, 163.63% relative enzyme activity in salivary glands | Chilo suppressalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 46.49% residual activity of the enzyme from midgut at 4 mM EDTA, 29.8% residual activity of the enzyme from salivary gland at 4 mM EDTA; 4 mmol/l, 46.49% relative enzyme activity in midgut, 29.8% relative enzyme activity in salivary glands | Chilo suppressalis | |
K+ | 58.03% residual activity of the enzyme from midgut at 40 mM K+, 45.95% residual activity of the enzyme from salivary gland at 40 mM K+ | Chilo suppressalis | |
KCl | 40 mmol/l, 58.03% relative enzyme activity in midgut, 45.95% relative enzyme activity in salivary glands | Chilo suppressalis | |
SDS | 61.32% residual activity of the enzyme from midgut at 8 mM SDS, 43.75% residual activity of the enzyme from salivary gland at 8 mM SDS; 8 mmol/l, 61.32% relative enzyme activity in midgut, 43.75% relative enzyme activity in salivary glands | Chilo suppressalis | |
Urea | 53.8% residual activity of the enzyme from midgut at 4 M urea, 20.05% residual activity of the enzyme from salivary gland at 6 M urea; 6000 mmol/l, 56.41% relative enzyme activity in midgut, 20.05% relative enzyme activity in salivary glands | Chilo suppressalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
4-nitrophenyl-beta-D-glucopyranoside | enzyme from salivary gland, in 100 mM universal buffer (pH 5.0) at 37°C | Chilo suppressalis | |
0.61 | - |
4-nitrophenyl-beta-D-glucopyranoside | enzyme from midgut, in 100 mM universal buffer (pH 5.0) at 37°C | Chilo suppressalis | |
0.61 | - |
p-nitrophenyl-beta-D-glucopyranoside | midgut | Chilo suppressalis | |
3.39 | - |
p-nitrophenyl-beta-D-glucopyranoside | salivary glands | Chilo suppressalis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 233.33% relative activity of the enzyme from midgut at 40 mM Ca2+, 193.31% relative activity of the enzyme from salivary gland at 40 mM Ca2+ | Chilo suppressalis | |
Mg2+ | 200% relative activity of the enzyme from midgut at 40 mM Mg2+, 169.49% relative activity of the enzyme from salivary gland at 40 mM Mg2+ | Chilo suppressalis | |
Na+ | 198.57% relative activity of the enzyme from midgut at 40 mM Na+, 163.63% relative activity of the enzyme from salivary gland at 40 mM Na+ | Chilo suppressalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chilo suppressalis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Chilo suppressalis | - |
midgut | - |
Chilo suppressalis | - |
salivary gland | - |
Chilo suppressalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000003 | - |
in salivary gland | Chilo suppressalis |
0.000005 | - |
in midgut | Chilo suppressalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Chilo suppressalis | 4-nitrophenol + D-glucopyranose | - |
? | |
p-nitrophenyl-beta-D-glucopyranoside + H2O | assay at pH 5.0, 37°C, reaction stopped by adding sodium carbonate | Chilo suppressalis | p-nitrophenol + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Chilo suppressalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Chilo suppressalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | activity increases steadily from 20-45°C | Chilo suppressalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Chilo suppressalis |
9 | - |
- |
Chilo suppressalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH | Chilo suppressalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | increase of enzyme activity with pH increase, above 8 decrease of enzyme activity with increasing pH | Chilo suppressalis |