Literature summary for 3.2.1.21 extracted from

Krogh, K.B.; Harris, P.V.; Olsen, C.L.; Johansen, K.S.; Hojer-Pedersen, J.; Borjesson, J.; Olsson, L.
Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum (2010), Appl. Microbiol. Biotechnol., 86, 143-154.

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Aspergillus oryzae	Penicillium brasilianum

Inhibitors

Inhibitors	Comment	Organism	Structure
4-nitrophenyl-beta-D-glucopyranoside	substrate inhibition	Penicillium brasilianum
D-glucono-delta-lactone	-	Penicillium brasilianum
D-glucose	-	Penicillium brasilianum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	4-nitrophenyl-beta-D-glucopyranoside	recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum
1.58	-	cellobiose	recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
115000	-	SDS-PAGE	Penicillium brasilianum

Organism

Organism	UniProt	Comment	Textmining
Penicillium brasilianum	A5A4M8	-	-
Penicillium brasilianum IBT 20888	A5A4M8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose column chromatography, Superdex 75 gel filtration, Resource Q column chromatography, Source 30S column chromatography, Superdex 200 gel filtration, and phenyl Sepharose column chromatography	Penicillium brasilianum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Penicillium brasilianum	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Penicillium brasilianum IBT 20888	4-nitrophenol + D-glucopyranose	-	?
cellobiose + H2O	BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside	Penicillium brasilianum	2 beta-D-glucose	-	?
cellobiose + H2O	BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside	Penicillium brasilianum IBT 20888	2 beta-D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
Bgl	-	Penicillium brasilianum
GH3 beta-glucosidase	-	Penicillium brasilianum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	at pH values 4.16, 4.80 and 6.17	Penicillium brasilianum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	no loss in activity after 24 h of incubation at 60°C at pH 4-6	Penicillium brasilianum

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
43	-	cellobiose	recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum
118	-	4-nitrophenyl-beta-D-glucopyranoside	recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.8	-	-	Penicillium brasilianum

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	6	stable in the range of pH 4.0 to 6.0 while outside this pH range no residual activity is measured, at 65°C the residual activity is 50% at pH 5.0 and 6.0 (after 24 h) and 20% at pH 4.0	Penicillium brasilianum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.012	-	D-glucono-delta-lactone	recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum
0.1	-	D-glucono-delta-lactone	recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum
1.1	-	D-glucose	recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum
2.3	-	D-glucose	recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C	Penicillium brasilianum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Penicillium brasilianum	isoelectric focusing	-	3.9

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Release 2023.1 (January 2023)