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Literature summary for 3.2.1.21 extracted from

  • Hu, Y.; Luan, H.; Hao, D.; Xiao, H.; Yang, S.; Yang, L.
    Purification and characterization of a novel ginsenoside-hydrolyzing beta-D-glucosidase from the China white jade snail (Achatina fulica) (2007), Enzyme Microb. Technol., 40, 1358-1366.
No PubMed abstract available
Search for more literature for 3.2.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.224
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 50°C Lissachatina fulica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 2 * 110000, SDS-PAGE Lissachatina fulica
220000
-
 gel filtration Lissachatina fulica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ginsenoside Rb1 + 3 H2O Lissachatina fulica
-
 (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose
-
 ?
ginsenoside Rb2 + 2 H2O Lissachatina fulica
-
 (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose
-
 ?
ginsenoside Rb3 + H2O Lissachatina fulica
-
 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose
-
 ?
ginsenoside Rc + H2O Lissachatina fulica
-
 (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Lissachatina fulica
-
 China white jade snail
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Lissachatina fulica

Purification (Commentary)

Purification (Comment) Organism
native enzyme 626fold from acetone powder of viscera by two steps of anion exchange chromatography, hydrophobic interaction chromatography, and two steps of gel filtration Lissachatina fulica

Source Tissue

Source Tissue Comment Organism Textmining
viscus
-
 Lissachatina fulica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
162.8
-
 purified enzyme Lissachatina fulica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O best substrate Lissachatina fulica 2-nitrophenol + beta-D-glucose
-
 ?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O high activity Lissachatina fulica 4-methylumbelliferol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O high activity Lissachatina fulica 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Lissachatina fulica 2 beta-D-glucose
-
 ?
gentiobiose + H2O
-
 Lissachatina fulica 2 beta-D-glucose
-
 ?
ginsenoside Rb1 + 3 H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose
-
 ?
ginsenoside Rb1 + 3 H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl beta-D-glucopyranoside + 3 beta-D-glucose i.e. compound K ?
ginsenoside Rb2 + 2 H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose
-
 ?
ginsenoside Rb2 + 2 H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside + 2 beta-D-glucose i.e. compound Y ?
ginsenoside Rb3 + H2O
-
 Lissachatina fulica 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose
-
 ?
ginsenoside Rb3 + H2O
-
 Lissachatina fulica 20-O-beta -D-xylopyranosyl-1,6-beta-D-glucopyranosyl-20(S)-protopanaxadiol + beta-D-glucose i.e. compound Mx ?
ginsenoside Rc + H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose
-
 ?
ginsenoside Rc + H2O
-
 Lissachatina fulica (3beta,5xi,6alpha,12beta,13xi)-3,6,12-trihydroxydammar-24-en-20-yl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranoside + beta-D-glucose i.e. compound Mc ?
additional information substrate specificity with various 4-nitrophenol glycosides, overview, poor activity with lactose, sucrose, trehalose, and ginsenoside Rd, the enzyme can cleave both beta-1,2-glucosidic linkage at 3-C and beta-1,6-glucosidic linkage at 20-C of ginsenosides and can hydrolyze ginsenosides Rb1, Rb2, Rb3 and Rc into their active metabolites, compound K, compound Y, Mx, and Mc, respectively Lissachatina fulica ?
-
 ?
sophorose + H2O
-
 Lissachatina fulica 2 beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 110000, SDS-PAGE Lissachatina fulica

Synonyms

Synonyms Comment Organism
ginsenoside-hydrolyzing beta-D-glucosidase
-
 Lissachatina fulica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lissachatina fulica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for 8 h Lissachatina fulica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Lissachatina fulica

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 30°C, stable for 24 h Lissachatina fulica