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Literature summary for 3.2.1.21 extracted from

  • Kaur, J.; Chadha, B.S.; Kumar, B.A.; Kaur, G.S.; Saini, H.S.
    Purification and characterization of beta-glucosidase from Melanocarpus sp. MTCC 3922 (2007), Electron. J. Biotechnol., 10, 260-270.
No PubMed abstract available
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
 Melanocarpus sp.
DTT
-
 Melanocarpus sp.
ethanol
-
 Melanocarpus sp.
methanol
-
 Melanocarpus sp.

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Melanocarpus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C Melanocarpus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Melanocarpus sp.
K+ activates Melanocarpus sp.
Mg2+ activates Melanocarpus sp.
Na+ activates Melanocarpus sp.
Zn2+ activates Melanocarpus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
 1 * 92000, SDS-PAGE Melanocarpus sp.
102000
-
 gel filtration Melanocarpus sp.

Organism

Organism UniProt Comment Textmining
Melanocarpus sp.
-
 thermophilic fungus
-
Melanocarpus sp. MTCC 3922
-
 thermophilic fungus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme to homogeneity Melanocarpus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + 2 H2O low activity Melanocarpus sp. 4-nitrophenol + 2 beta-D-glucose
-
 ?
4-nitrophenyl beta-D-cellobioside + 2 H2O low activity Melanocarpus sp. MTCC 3922 4-nitrophenol + 2 beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Melanocarpus sp. 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Melanocarpus sp. MTCC 3922 4-nitrophenol + beta-D-glucose
-
 ?
additional information the enzyme exhibits transglycosylation activity with methanol as acceptor Melanocarpus sp. ?
-
 ?
additional information the enzyme exhibits transglycosylation activity with methanol as acceptor Melanocarpus sp. MTCC 3922 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 92000, SDS-PAGE Melanocarpus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Melanocarpus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 65
-
 Melanocarpus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, stable Melanocarpus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
66.67
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C Melanocarpus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Melanocarpus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 6 purified enzyme, stable Melanocarpus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Melanocarpus sp.
-
-
 4.1