Literature summary for 3.2.1.21 extracted from

Barrera-Islas, G.A.; Ramos-Valdivia, A.C.; Salgado, L.M.; Ponce-Noyola, T.
Characterization of a beta-glucosidase produced by a high-specific growth-rate mutant of Cellulomonas flavigena (2007), Curr. Microbiol., 54, 266-270.

Search for more literature for 3.2.1.21

Protein Variants

Protein Variants	Comment	Organism
additional information	mutant strain PN-120 produces a beta-glucosidase that is 10fold more active with a 3fold higher affinity for cellobiose than the corresponding enzyme isolated from the parental strain, the mutation of strain PN-120 affects the catalytic site	Cellulomonas flavigena

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
5.25	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.2, 40°C, mutant enzyme	Cellulomonas flavigena
7.1	-	4-nitrophenyl-beta-D-glucopyranoside	pH 6.2, 40°C, wild-type enzyme	Cellulomonas flavigena
37	-	cellobiose	pH 6.2, 40°C, mutant enzyme	Cellulomonas flavigena
114	-	cellobiose	pH 6.2, 40°C, wild-type enzyme	Cellulomonas flavigena

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
210000	-	gel filtration	Cellulomonas flavigena

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose + H2O	Cellulomonas flavigena	-	2 beta-D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Cellulomonas flavigena	-	wild-type strain CDBB531 and mutant strain PN-120	-

Purification (Commentary)

Purification (Comment)	Organism
native wild-type and mutant enzymes partially by anion exchange chromatography and gel filtration 29fold and 16fold, respectively	Cellulomonas flavigena

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	mutant strain PN-120 produces a beta-glucosidase that is 16fold more active than the corresponding enzyme isolated from the parental strain	Cellulomonas flavigena	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.27	-	partially purified wild-type enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside	Cellulomonas flavigena
14.1	-	partially purified mutant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside	Cellulomonas flavigena

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O	-	Cellulomonas flavigena	4-methylumbelliferol + beta-D-glucose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Cellulomonas flavigena	4-nitrophenol + beta-D-glucose	-	?
cellobiose + H2O	-	Cellulomonas flavigena	2 beta-D-glucose	-	?

Subunits

Subunits	Comment	Organism
oligomer	three protein bands in SDS-PAGE	Cellulomonas flavigena

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	40	wild-type and mutant enzymes	Cellulomonas flavigena

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	70	-	Cellulomonas flavigena

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	wild-type and mutant enzymes	Cellulomonas flavigena

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	-	Cellulomonas flavigena

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Release 2023.1 (January 2023)