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Literature summary for 3.2.1.21 extracted from

  • Saibi, W.; Amouri, B.; Gargouri, A.
    Purification and biochemical characterization of a transglucosilating beta-glucosidase of Stachybotrys strain (2007), Appl. Microbiol. Biotechnol., 77, 293-300.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ activates at 5 mM, but shows 50% inhibition at 10 mM Stachybotrys sp.
additional information optimal activity is obtained with 100 mM phosphate buffer and, the activity decreased at 150 and 200 mM but not dramatically Stachybotrys sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.85
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 50°C Stachybotrys sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Stachybotrys sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates 1.4fold at 10 mM Stachybotrys sp.
Ca2+ activates Stachybotrys sp.
Fe2+ activates at 5 mM, but shows 50% inhibition at 10 mM Stachybotrys sp.
additional information no effect by Co2+, optimal activity is obtained with 100 mM phosphate buffer and, the activity decreased at 150 and 200 mM but not dramatically Stachybotrys sp.
Zn2+ activates Stachybotrys sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 gel filtration Stachybotrys sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Stachybotrys sp. the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, involvement of a transglucosylation to synthesize cellotetraose by a sequential addition of glucose to cellotriose, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Stachybotrys sp.
-
 a filamentous fungus, strain A19, isolated after nitrous acid mutagenesis from the cellulolytic fungus N1, five isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular isozyme by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Stachybotrys sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-methylumbelliferol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Stachybotrys sp. 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Stachybotrys sp. 2 beta-D-glucose
-
 ?
additional information the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, involvement of a transglucosylation to synthesize cellotetraose by a sequential addition of glucose to cellotriose, overview Stachybotrys sp. ?
-
 ?
additional information substrate specificity, overview, no activity with 4-methylumbelliferyl-beta-D-cellobioside, the enzyme has the capacity to synthesize short oligosaccharides from cellobiose as the substrate concentration reaches 30% with a recovery of 40%, overview Stachybotrys sp. ?
-
 ?
salicin + H2O
-
 Stachybotrys sp. ? + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE Stachybotrys sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Stachybotrys sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
915
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 50°C Stachybotrys sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Stachybotrys sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 stable Stachybotrys sp.