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Literature summary for 3.2.1.21 extracted from

  • Mamma, D.; Hatzinikolaou, D.G.; Christakopoulos, P.
    Biochemical and catalytic properties of two intracellular beta-glucosidases from the fungus Penicillium decumbens active on flavonoid glucosides (2004), J. Mol. Catal. B, 27, 183-190.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 110 mM, more than 90% inhibition Penicillium decumbens
glucono-delta-lactone 5.6 mM, more than 90% inhibition Penicillium decumbens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.5 Penicillium decumbens
0.013
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.5 Penicillium decumbens
0.11
-
 arbutin 40°C, pH 6.5 Penicillium decumbens
0.13
-
 amygdalin 40°C, pH 6.5 Penicillium decumbens
0.23
-
 arbutin 40°C, pH 6.5 Penicillium decumbens
0.36
-
 gentiobiose 40°C, pH 6.5 Penicillium decumbens
0.4
-
 cellobiose 40°C, pH 6.5 Penicillium decumbens
0.4
-
 apigetrin 40°C, pH 6.5 Penicillium decumbens
0.45
-
 naringenin 40°C, pH 6.5 Penicillium decumbens
0.46
-
 Salicin 40°C, pH 6.5 Penicillium decumbens
0.48
-
 cellobiose 40°C, pH 6.5 Penicillium decumbens
0.49
-
 Salicin 40°C, pH 6.5 Penicillium decumbens
0.54
-
 apigetrin 40°C, pH 6.5 Penicillium decumbens
0.65
-
 daidzin 40°C, pH 6.5 Penicillium decumbens
0.67
-
 naringenin 40°C, pH 6.5 Penicillium decumbens
0.83
-
 gentiobiose 40°C, pH 6.5 Penicillium decumbens
0.97
-
 daidzin 40°C, pH 6.5 Penicillium decumbens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Penicillium decumbens
50000
-
 native-PAGE Penicillium decumbens
115000
-
 4 * 115000, SDS-PAGE Penicillium decumbens
460000
-
 native-PAGE Penicillium decumbens

Organism

Organism UniProt Comment Textmining
Penicillium decumbens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Q-Sepharose, DEAE-Toyopearl, t-Butyl Penicillium decumbens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.15
-
-
 Penicillium decumbens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Penicillium decumbens 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O 6% of activity with p-nitrophenyl-beta-D-glucopyranoside Penicillium decumbens 4-nitrophenol + D-glucose
-
 ?
amygdalin + 2 H2O 68% of activity with gentiobiose Penicillium decumbens mandelonitrile + 2 beta-D-glucose
-
 ?
amygdalin + 2 H2O 80% of activity with gentiobiose Penicillium decumbens mandelonitrile + 2 beta-D-glucose
-
 ?
apigetrin + H2O
-
 Penicillium decumbens beta-D-glucose + apigenin
-
 ?
arbutin + H2O 10% of activity with gentiobiose Penicillium decumbens hydroquinol + beta-D-glucose
-
 ?
arbutin + H2O 8% of activity with gentiobiose Penicillium decumbens hydroquinol + beta-D-glucose
-
 ?
cellobiose + H2O 28% of activity with gentiobiose Penicillium decumbens 2 beta-D-glucose
-
 ?
cellobiose + H2O 74% of activity with gentiobiose Penicillium decumbens 2 beta-D-glucose
-
 ?
daidzin + H2O
-
 Penicillium decumbens beta-D-glucose + daidzein
-
 ?
gentiobiose + H2O
-
 Penicillium decumbens 2 beta-D-glucose
-
 ?
laminarin + H2O 39% of activity with gentiobiose Penicillium decumbens beta-D-glucose
-
 ?
laminarin + H2O 54% of activity with gentiobiose Penicillium decumbens beta-D-glucose
-
 ?
lichenan + H2O 23% of activity with gentiobiose Penicillium decumbens beta-D-glucose
-
 ?
lichenan + H2O 47% of activity with gentiobiose Penicillium decumbens beta-D-glucose
-
 ?
naringenin + H2O
-
 Penicillium decumbens beta-D-glucose + naringenin
-
 ?
p-nitrophenyl-beta-D-cellobioside + H2O 17% of activity with p-nitrophenyl-beta-d-glucopyranoside Penicillium decumbens p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-cellobioside + H2O 22% of activity with p-nitrophenyl-beta-D-glucopyranoside Penicillium decumbens p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Penicillium decumbens p-nitrophenol + beta-D-glucose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O 13% of activity with p-nitrophenyl-beta-D-glucopyranoside Penicillium decumbens p-nitrophenol + beta-D-xylose
-
 ?
p-nitrophenyl-beta-D-xylopyranoside + H2O 14% of activity with p-nitrophenyl-beta-D-glucopyranoside Penicillium decumbens p-nitrophenol + beta-D-xylose
-
 ?
salicin + H2O 36% of activity with gentiobiose Penicillium decumbens salicyl alcohol + beta-D-glucose
-
 ?
salicin + H2O 81% of activity with gentiobiose Penicillium decumbens salicyl alcohol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 28000, SDS-PAGE Penicillium decumbens
tetramer 4 * 115000, SDS-PAGE Penicillium decumbens

Synonyms

Synonyms Comment Organism
GI
-
 Penicillium decumbens
GII
-
 Penicillium decumbens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Penicillium decumbens
75
-
-
 Penicillium decumbens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 complete loss of activity after 1 h Penicillium decumbens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
 arbutin 40°C, pH 6.5 Penicillium decumbens
0.1
-
 cellobiose 40°C, pH 6.5 Penicillium decumbens
0.1
-
 Salicin 40°C, pH 6.5 Penicillium decumbens
0.1
-
 arbutin 40°C, pH 6.5 Penicillium decumbens
0.17
-
 apigetrin 40°C, pH 6.5 Penicillium decumbens
0.19
-
 naringenin 40°C, pH 6.5 Penicillium decumbens
0.2
-
 daidzin 40°C, pH 6.5 Penicillium decumbens
0.36
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.5 Penicillium decumbens
0.4
-
 gentiobiose 40°C, pH 6.5 Penicillium decumbens
0.4
-
 daidzin 40°C, pH 6.5 Penicillium decumbens
0.5
-
 cellobiose 40°C, pH 6.5 Penicillium decumbens
0.6
-
 naringenin 40°C, pH 6.5 Penicillium decumbens
0.6
-
 gentiobiose 40°C, pH 6.5 Penicillium decumbens
0.6
-
 apigetrin 40°C, pH 6.5 Penicillium decumbens
0.7
-
 Salicin 40°C, pH 6.5 Penicillium decumbens
1.5
-
 p-nitrophenyl-beta-D-glucopyranoside 40°C, pH 6.5 Penicillium decumbens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Penicillium decumbens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0005
-
 glucono-delta-lactone 40°C, pH 6.0 Penicillium decumbens
0.0006
-
 glucono-delta-lactone 40°C, pH 6.0 Penicillium decumbens
0.24
-
 D-glucose 40°C, pH 6.0 Penicillium decumbens
0.29
-
 D-glucose 40°C, pH 6.0 Penicillium decumbens