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Literature summary for 3.2.1.21 extracted from
- Beta-glucosidase from Chalara paradoxa CH32: purification and properties (2000), J. Agric. Food Chem., 48, 3698-3703.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | 20%, 176% activation | Thielaviopsis paradoxa |  |
| dimethyl sulfoxide | 20%, 92% activation | Thielaviopsis paradoxa | |
| ethanol | 20%, 197% activation | Thielaviopsis paradoxa | |
| ethyl acetate | 20%, 171% activation | Thielaviopsis paradoxa | |
| methanol | 20%, 168% activation | Thielaviopsis paradoxa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | 10 mM, 84% inhibition | Thielaviopsis paradoxa | |
| D-delta-gluconolactone | complete inhibition | Thielaviopsis paradoxa | |
| D-glucose | - |
Thielaviopsis paradoxa | |
| HgCl2 | 1 mM, 24% inhibition, 10 mM, 99% inhibition | Thielaviopsis paradoxa | |
| Sodium dodecyl sulfate | 0.1%, 22% inhibition | Thielaviopsis paradoxa | |
| Tween 80 | 0.1%, 40% inhibition | Thielaviopsis paradoxa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
p-nitrophenyl-beta-D-glucopyranoside | 40°C, pH 5.0 | Thielaviopsis paradoxa | |
| 0.58 | - |
cellobiose | 40°C, pH 5.0 | Thielaviopsis paradoxa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thielaviopsis paradoxa | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 167000 | - |
1 * 167000, SDS-PAGE | Thielaviopsis paradoxa |
| 170000 | - |
gel filtration | Thielaviopsis paradoxa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thielaviopsis paradoxa | - |
- |
- |
| Thielaviopsis paradoxa CH32 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE A-50, Sephacryl S-200, Mono Q, TSK DEAE-5PW, Superose 12 | Thielaviopsis paradoxa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | 89% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa | 2 beta-D-glucose | - |
? | |
| cellobiose + H2O | 89% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa CH32 | 2 beta-D-glucose | - |
? | |
| esculin + H2O | 87% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa | beta-D-glucose + esculetin | - |
? | |
| esculin + H2O | 87% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa CH32 | beta-D-glucose + esculetin | - |
? | |
| p-nitrophenyl-beta-D-cellobioside | 8% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa | p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-cellobioside | 8% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa CH32 | p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thielaviopsis paradoxa | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Thielaviopsis paradoxa CH32 | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucosamine | 27% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa | p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucosamine | - |
? | |
| p-nitrophenyl-beta-D-glucosamine | 27% of activity with p-nitrophenyl-beta-D-glucose | Thielaviopsis paradoxa CH32 | p-nitrophenyl-beta-D-glucopyranoside + beta-D-glucosamine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 167000, SDS-PAGE | Thielaviopsis paradoxa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Thielaviopsis paradoxa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | approx. 20% of maximal activity at 10°C and 60°C, respecttively | Thielaviopsis paradoxa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 40 | no loss of activity after 30 min | Thielaviopsis paradoxa |
| 50 | - |
approx. 55 loss of activity after 30 min | Thielaviopsis paradoxa |
| 60 | - |
approx. 80% loss of activity after 30 min | Thielaviopsis paradoxa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Thielaviopsis paradoxa |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | approx. 60% of maximal activity at pH 3.0, approx. 5% of maximal activity at pH 9.0 | Thielaviopsis paradoxa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5 | approx. 80% loss of activity after 1 h at pH 3.0 and 28°C | Thielaviopsis paradoxa |
| 6 | 9 | no loss of activity after 1 h at 28°C | Thielaviopsis paradoxa |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.02 | - |
D-glucose | 40°C, pH 5.0 | Thielaviopsis paradoxa | |
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