Any feedback?
Literature summary for 3.2.1.21 extracted from
- Purification and characterization of beta-glucosidase from honey bees (Apis mellifera) (2002), Insect Biochem. Mol. Biol., 32, 679-690.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.3 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from honey sac | Apis mellifera |  |
| 12 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from ventriculus | Apis mellifera | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
x * 72000, SDS-PAGE | Apis mellifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Apis mellifera | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Apis mellifera |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose, Superose, CM-sepharose, Mono S, hydroxyapatite, Superose 12 | Apis mellifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| honey sac | - |
Apis mellifera | - |
| ventriculus | - |
Apis mellifera | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.49 | - |
beta-glucosidase purified from honey sac | Apis mellifera |
| 5.66 | - |
beta-glucosidase purified from ventriculus | Apis mellifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Apis mellifera | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, SDS-PAGE | Apis mellifera |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | activity slowly increases as a function of temperature | Apis mellifera |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | no loss of activity after 20 min | Apis mellifera |
| 55 | - |
approx. 80% loss of activity after 20 min | Apis mellifera |
| 60 | - |
complete loss of activity after 20 min | Apis mellifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Apis mellifera |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | increase in activity until pH 5.5, sharp drop in activity above pH 6.0 | Apis mellifera |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 9.5 | no loss of activity after 20 min at 25°C | Apis mellifera |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Apis mellifera | isoelectric focusing, different isoenzymes probably due to different degrees of glycosylation | - |
4.5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
