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Literature summary for 3.2.1.21 extracted from

  • Kawai, R.; Yoshida, M.; Tani, T.; Igarashi, K.; Ohira, T.; Nagasawa, H.; Samejima, M.
    Production and characterization of recombinant Phanerochaete chrysosporium beta-glucosidase in the methylotrophic yeast Pichia pastoris (2003), Biosci. Biotechnol. Biochem., 67, 1-7.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
delta-gluconolactone
-
Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
p-nitrophenyl-beta-D-glucose wild-type BGL Phanerodontia chrysosporium
0.2
-
p-nitrophenyl-beta-D-glucose recombinant BGL Phanerodontia chrysosporium
4.06
-
cellobiose wild-type BGL Phanerodontia chrysosporium
5.05
-
cellobiose recombinant BGL Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
x * 116000, SDS-PAGE, wild-type BGL Phanerodontia chrysosporium
133000
-
x * 133000, SDS-PAGE, recombinant BGL Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium Q8TGC6 white-rot fungus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant and wild-type beta-glucosidase Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose + H2O
-
Phanerodontia chrysosporium 2 beta-D-glucose
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
Phanerodontia chrysosporium p-nitrophenol + beta-D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 116000, SDS-PAGE, wild-type BGL Phanerodontia chrysosporium
? x * 133000, SDS-PAGE, recombinant BGL Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
Bgl
-
Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
27.5
-
cellobiose wild-type BGL Phanerodontia chrysosporium
29.8
-
cellobiose recombinant BGL Phanerodontia chrysosporium
96.2
-
p-nitrophenyl-beta-D-glucose wild-type BGL Phanerodontia chrysosporium
103.1
-
p-nitrophenyl-beta-D-glucose recombinant BGL Phanerodontia chrysosporium

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2
-
delta-gluconolactone hydrolysis of p-nitrophenyl-beta-D-glucopyranoside Phanerodontia chrysosporium