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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of two beta-glucosidases from a thermo-tolerant yeast Pichia etchellsii (2003), Biochim. Biophys. Acta, 1649, 74-84.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | 10% stimulation at 4%, inhibition above | Schwanniomyces etchellsii |  |
| methanol | 15% stimulation at 4%, inhibition above | Schwanniomyces etchellsii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-propanol | inhibition above 4% | Schwanniomyces etchellsii | |
| beta-D-glucose | - |
Schwanniomyces etchellsii | |
| cellobiose | substrate inhibition above 100 mM | Schwanniomyces etchellsii | |
| Cu2+ | 1 mM, 70% inhibition | Schwanniomyces etchellsii | |
| D-glucose | - |
Schwanniomyces etchellsii | |
| Fe2+ | 1 mM, 40% inhibition | Schwanniomyces etchellsii | |
| gluconolactone | - |
Schwanniomyces etchellsii | |
| glucose | - |
Schwanniomyces etchellsii | |
| Hg2+ | 1 mM, complete inhibition, 73% reversion by addition of 10 mM dithiothreitol | Schwanniomyces etchellsii | |
| methanol | inhibition above 4% | Schwanniomyces etchellsii | |
| p-chloromercuribenzoate | 1 mM, complete inhibition | Schwanniomyces etchellsii | |
| p-nitrophenyl-beta-D-glucopyranoside | substrate inhibition above 5 mM | Schwanniomyces etchellsii | |
| sodium dodecylsulfate | 1 mM, complete inactivation | Schwanniomyces etchellsii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
p-nitrophenyl-beta-D-glucopyranoside | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 24 | - |
cellobiose | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
4 * 90000, SDS-PAGE | Schwanniomyces etchellsii |
| 102000 | - |
2 * 102000, SDS-PAGE | Schwanniomyces etchellsii |
| 186000 | - |
gel filtration | Schwanniomyces etchellsii |
| 340000 | - |
gel filtration | Schwanniomyces etchellsii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schwanniomyces etchellsii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sepharose, hydroxyapatite | Schwanniomyces etchellsii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.6 | - |
- |
Schwanniomyces etchellsii |
| 35 | - |
- |
Schwanniomyces etchellsii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM phosphate buffer, no loss of activity | Schwanniomyces etchellsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-amygdalin + H2O | - |
Schwanniomyces etchellsii | beta-D-glucose + ? | - |
? | |
| cellobiose + H2O | - |
Schwanniomyces etchellsii | 2 beta-D-glucose | - |
? | |
| gentiobiose + H2O | - |
Schwanniomyces etchellsii | 2 beta-D-glucose | - |
? | |
| hexyl-beta-D-glucoside + H2O | - |
Schwanniomyces etchellsii | hexanol + beta-D-glucose | - |
? | |
| laminaribiose + H2O | - |
Schwanniomyces etchellsii | 2 beta-D-glucose | - |
? | |
| methyl-beta-D-glucoside + H2O | - |
Schwanniomyces etchellsii | methanol + beta-D-glucose | - |
? | |
| octyl-beta-D-glucoside + H2O | - |
Schwanniomyces etchellsii | octanol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | substrate specificity in descending order, arylglucosides, alkylglucosides and disaccharides | Schwanniomyces etchellsii | p-nitrophenol + beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-xylopyranoside + H2O | - |
Schwanniomyces etchellsii | p-nitrophenol + beta-D-xylose | - |
? | |
| phenyl-beta-D-glucopyranoside + H2O | - |
Schwanniomyces etchellsii | phenol + beta-D-glucose | - |
? | |
| salicin + H2O | - |
Schwanniomyces etchellsii | saligenin + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 102000, SDS-PAGE | Schwanniomyces etchellsii |
| tetramer | 4 * 90000, SDS-PAGE | Schwanniomyces etchellsii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BglI | - |
Schwanniomyces etchellsii |
| BglII | - |
Schwanniomyces etchellsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Schwanniomyces etchellsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
no loss of activity after 8 h | Schwanniomyces etchellsii |
| 45 | - |
50% loss of activity after 3.4 h | Schwanniomyces etchellsii |
| 45 | - |
50% loss of activity after 3.5 h | Schwanniomyces etchellsii |
| 50 | - |
50% loss of activity after 15 min | Schwanniomyces etchellsii |
| 50 | - |
50% loss of activity after 14 min | Schwanniomyces etchellsii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 141 | - |
p-nitrophenyl-beta-D-glucopyranoside | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 170 | - |
p-nitrophenyl-beta-D-glucopyranoside | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 233 | - |
cellobiose | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Schwanniomyces etchellsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 40% loss of activity after 24 h at 0°C and pH 3.0, approx. 20% loss of activity after 24 h at 0°C and pH 4.0-9.0 | Schwanniomyces etchellsii |
| 3 | 9 | 40% loss of activity after 24 h at 0°C and pH 3.0, approx. 205 loss of activity after 24 h at 0°C and pH 4.0-9.0 | Schwanniomyces etchellsii |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
gluconolactone | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 0.12 | - |
gluconolactone | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 2 | 5 | beta-D-glucose | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
| 12 | - |
beta-D-glucose | 50°C, pH 6.0 | Schwanniomyces etchellsii | |
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