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Literature summary for 3.2.1.21 extracted from
- Production, purification, and biochemical characterization of two beta-glucosidases from Sclerotinia sclerotiorum (2003), Appl. Biochem. Biotechnol., 111, 29-40.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Dimethylsulfoxide | 2 mM, 23% activation | Sclerotinia sclerotiorum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 5 mM, 90% inhibition | Sclerotinia sclerotiorum | |
| Fe2+ | 5 mM, 97% inhibition | Sclerotinia sclerotiorum | |
| Zn2+ | 5 mM, 43% inhibition | Sclerotinia sclerotiorum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
p-nitrophenyl-beta-D-glucopyranoside | 60°C, pH 5.0 | Sclerotinia sclerotiorum | |
| 2.8 | - |
cellobiose | 60°C, pH 5.0 | Sclerotinia sclerotiorum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 5 mM, 21% activation | Sclerotinia sclerotiorum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96500 | - |
2 * 96500, SDS-PAGE | Sclerotinia sclerotiorum |
| 196000 | - |
gel filtration | Sclerotinia sclerotiorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | Sclerotinia sclerotiorum | - |
2 beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sclerotinia sclerotiorum | - |
filamentous fungi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sepharose, TSK-DEAE-5PW, TSK 3000 SW | Sclerotinia sclerotiorum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 277.5 | - |
- |
Sclerotinia sclerotiorum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | - |
Sclerotinia sclerotiorum | 2 beta-D-glucose | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Sclerotinia sclerotiorum | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 96500, SDS-PAGE | Sclerotinia sclerotiorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glu1 | - |
Sclerotinia sclerotiorum |
| beta-glu2 | - |
Sclerotinia sclerotiorum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Sclerotinia sclerotiorum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
50% loss of activity after 30 min | Sclerotinia sclerotiorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
at 60°C | Sclerotinia sclerotiorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | no loss of activity | Sclerotinia sclerotiorum |
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