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Literature summary for 3.2.1.21 extracted from
- Purification, characterization, and properties of beta-glucosidase enzymes from Sclerotium rolfsii (1981), Arch. Biochem. Biophys., 207, 185-196.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | Ki: 0.55 mM | Athelia rolfsii |  |
| D-glucono-1,5-lactone | Ki: 0.01 mM | Athelia rolfsii | |
| nojirimycin bisulfite | Ki: 0.001 mM | Athelia rolfsii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.79 | 1.38 | 4-nitrophenyl-beta-D-glucopyranoside | 4 different isoforms | Athelia rolfsii | |
| 3.07 | 5.84 | cellobiose | 4 different isoforms, Km depends on pH-value, ionizing groups control binding of substrate | Athelia rolfsii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Athelia rolfsii | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
beta-glucosidases 1 and 2, gel filtration | Athelia rolfsii |
| 107000 | - |
beta-glucosidase 3, gel filtration | Athelia rolfsii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Athelia rolfsii | cellobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Athelia rolfsii | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains alpha-D-mannose-, alpha-D-glucose- and 2-O-D-mannose-residues | Athelia rolfsii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, 4 different forms, chromatography steps | Athelia rolfsii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | 4 different isoforms | Athelia rolfsii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 51 | 175 | 4 different isoforms, cellobiose as substrate | Athelia rolfsii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, pH 4.5, 12 months | Athelia rolfsii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Athelia rolfsii | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Athelia rolfsii | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Athelia rolfsii | 2 beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000-107000, SDS-PAGE, 4 isoforms | Athelia rolfsii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 68 | - |
Athelia rolfsii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
50% of activity remains after 10 min | Athelia rolfsii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | 4.5 | depending on substrate used | Athelia rolfsii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | most stable within | Athelia rolfsii |
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