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Literature summary for 3.2.1.21 extracted from

  • Dale, M.P.; Ensley, H.E.; Kern, K.; Sastry, K.A.R.; Byers, L.D.
    Reversible inhibitors of beta-glucosidase (1985), Biochemistry, 24, 3530-3539.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
1-deoxynojirimycin fairly potent inhibitor, Ki: 0.029 mM Prunus dulcis
2-deoxy-2-[(p-chlorobenzyl)amino]glucose Ki: 3 mM Prunus dulcis
3-deoxy-3-aminoglucose Ki: 8 mM Prunus dulcis
beta-D-glucose competitive inhibition, Ki: 189 mM Prunus dulcis
D-glucono-1,5-lactone fairly potent inhibitor, Ki: 0.2 mM Prunus dulcis
L-ascorbic acid Ki: 2 mM Prunus dulcis
N-methylglucamine Ki: 1 mM Prunus dulcis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.93
-
 4-nitrophenyl-beta-D-fucoside
-
 Prunus dulcis
2.5
-
 4-nitrophenyl-beta-D-glucoside
-
 Prunus dulcis
3.1
-
 4-nitrophenyl-beta-D-xyloside
-
 Prunus dulcis
15.7
-
 4-nitrophenyl-beta-D-galactoside
-
 Prunus dulcis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 native PAGE, SDS-PAGE Prunus dulcis

Organism

Organism UniProt Comment Textmining
Prunus dulcis
-
 var. sativa
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Prunus dulcis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O
-
 Prunus dulcis 4-nitrophenol + beta-D-fucose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Prunus dulcis 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Prunus dulcis 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Prunus dulcis 4-nitrophenol + beta-D-xylose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 135000, SDS-PAGE Prunus dulcis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Prunus dulcis