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Literature summary for 3.2.1.21 extracted from

  • Kantham, L.; Jagannathan, V.
    beta-Glucosidase of Penicillium funiculosum. II: Properties and mycelial binding (1985), Biotechnol. Bioeng., 23, 786-791.
No PubMed abstract available
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
Isopropanol 4fold activation by preincubation with 80%, v/v Talaromyces funiculosus

General Stability

General Stability Organism
bovine serum albumin required for stabilizing the enzyme preparation Talaromyces funiculosus
marked instability after purification Talaromyces funiculosus

Inhibitors

Inhibitors Comment Organism Structure
beta-D-glucose competitive inhibition, Ki: 0.9 mM Talaromyces funiculosus
D-glucono-1,5-lactone competitive inhibition, Ki: 0.03 mM Talaromyces funiculosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 4-nitrophenyl-beta-D-glucopyranoside isoform II Talaromyces funiculosus
0.002
-
 cellobiose
-
 Talaromyces funiculosus
0.002
-
 4-nitrophenyl-beta-D-glucopyranoside
-
 Talaromyces funiculosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular absorption of enzyme to mycelium, if cells grown on glucose medium Talaromyces funiculosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 gel filtration, isoform II Talaromyces funiculosus
120000
-
 2 * 120000, SDS-PAGE, isoform I Talaromyces funiculosus
230000
-
 gel filtration, isoform I Talaromyces funiculosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Talaromyces funiculosus cellobiose beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O Talaromyces funiculosus gentiobiose beta-D-glucose + D-glucose
-
 ?
cellopentaose + 4 H2O Talaromyces funiculosus
-
 5 D-glucose
-
 ?
cellotetraose + 3 H2O Talaromyces funiculosus
-
 4 D-glucose
-
 ?
cellotriose + 2 H2O Talaromyces funiculosus
-
 3 D-glucose
-
 ?
laminarin + H2O Talaromyces funiculosus low activity beta-D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Talaromyces funiculosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 10.7% carbohydrate content, w/w, isoform I Talaromyces funiculosus

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, preparative electrophoresis, 2 isoforms Talaromyces funiculosus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Talaromyces funiculosus
-

Storage Stability

Storage Stability Organism
-20°C, stable for months in solution Talaromyces funiculosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Talaromyces funiculosus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Talaromyces funiculosus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Talaromyces funiculosus 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Talaromyces funiculosus beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose Talaromyces funiculosus beta-D-glucose + D-glucose
-
 ?
cellopentaose + 4 H2O
-
 Talaromyces funiculosus 5 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Talaromyces funiculosus 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Talaromyces funiculosus 3 D-glucose
-
 ?
laminarin + H2O low activity Talaromyces funiculosus beta-D-glucose + ?
-
 ?
methyl-beta-D-glucoside + H2O very low activity Talaromyces funiculosus beta-D-glucose + methanol
-
 ?
phenyl-beta-D-glucoside + H2O
-
 Talaromyces funiculosus phenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 120000, SDS-PAGE, isoform I Talaromyces funiculosus