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Literature summary for 3.2.1.21 extracted from

  • Sanyal, A.; Kundu, R.K.; Dube, S.; Dube, D.S.
    Extracellular cellulolytic enzyme system of Aspergillus japonicus: 2. Purification and characterization of an inducible extracellular beta-glucosidase (1988), Enzyme Microb. Technol., 10, 91-99.
No PubMed abstract available
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone competitive inhibition, Ki: 0.04 mM Aspergillus japonicus
Hg2+ strong inhibition at 1 mM Aspergillus japonicus
nojirimycin bisulfite competitive inhibition, Ki: 0.0018 mM Aspergillus japonicus
p-chloromercuribenzoic acid strong inhibition at 1.25 mM Aspergillus japonicus
SDS 40% inhibition at 1%, w/v Aspergillus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.66
-
 amygdalin
-
 Aspergillus japonicus
1.17
-
 2-Nitrophenyl-beta-D-glucopyranoside
-
 Aspergillus japonicus
2.17
-
 Salicin
-
 Aspergillus japonicus
5.7
-
 cellobiose
-
 Aspergillus japonicus
166.7
-
 methyl-beta-D-glucoside
-
 Aspergillus japonicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus japonicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight stimulation Aspergillus japonicus
Mn2+ slight stimulation Aspergillus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 gel filtration Aspergillus japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-glucopyranosyl-D-glucose + H2O Aspergillus japonicus cellobiose beta-D-glucose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography Aspergillus japonicus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Aspergillus japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.5
-
-
 Aspergillus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Aspergillus japonicus 2-hydroxymethylphenol + beta-D-glucose
-
 ?
(R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O amygdalin Aspergillus japonicus HCN + ?
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Aspergillus japonicus 2-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Aspergillus japonicus beta-D-glucose + D-glucose
-
 ?
cellobiose + H2O
-
 Aspergillus japonicus 2 beta-D-glucose
-
 ?
methyl-beta-D-glucoside + H2O
-
 Aspergillus japonicus beta-D-glucose + methanol
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Aspergillus japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life: 15 min at pH 7.0, at pH 5.0 no decline of activity Aspergillus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus japonicus