Literature summary for 3.2.1.21 extracted from

Plant, A.R.; Oliver, J.E.; Patchett, M.L.; Daniel, R.M.; Morgan, H.W.
Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli (1988), Arch. Biochem. Biophys., 262, 181-188.

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Caldicellulosiruptor saccharolyticus

General Stability

General Stability	Organism
stable in the presence of cetyl-trimethylammonium bromide	Caldicellulosiruptor saccharolyticus
stable in the presence of urea	Caldicellulosiruptor saccharolyticus

Inhibitors

Inhibitors	Comment	Organism	Structure
1-O-methyl-beta-D-glucose	competitive inhibition, Ki: 17.5 mM	Caldicellulosiruptor saccharolyticus
Co2+	destabilizing effect	Caldicellulosiruptor saccharolyticus
D-glucono-1,4-lactone	Ki: 1.6 mM	Caldicellulosiruptor saccharolyticus
D-glucono-1,5-lactone	Ki: 0.09 mM	Caldicellulosiruptor saccharolyticus
Hg2+	strong inactivation at 0.5 mM, reversal by addition of 10 mM beta-mercaptoethanol	Caldicellulosiruptor saccharolyticus
La3+	rapid inactivation at 3 mM	Caldicellulosiruptor saccharolyticus
N-ethylmaleimide	rapid loss of activity at 5 mM, reversal by addition of beta-mercaptoethanol	Caldicellulosiruptor saccharolyticus
Ni2+	strong inhibition	Caldicellulosiruptor saccharolyticus
SDS	complete inhibition at 1 mg/ml	Caldicellulosiruptor saccharolyticus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.24	-	4-nitrophenyl-beta-D-fucoside	-	Caldicellulosiruptor saccharolyticus
0.29	-	2-nitrophenyl beta-D-glucopyranoside	-	Caldicellulosiruptor saccharolyticus
0.51	-	4-nitrophenyl-beta-D-glucopyranoside	-	Caldicellulosiruptor saccharolyticus
1.88	-	Salicin	-	Caldicellulosiruptor saccharolyticus
1.94	-	4-nitrophenyl-beta-D-galactopyranoside	-	Caldicellulosiruptor saccharolyticus
2.12	-	2-nitrophenyl beta-D-galactopyranoside	-	Caldicellulosiruptor saccharolyticus
3	-	laminaribiose	-	Caldicellulosiruptor saccharolyticus
5.2	-	sophorose	-	Caldicellulosiruptor saccharolyticus
6.93	-	4-nitrophenyl-beta-D-xyloside	-	Caldicellulosiruptor saccharolyticus
18.2	-	cellobiose	-	Caldicellulosiruptor saccharolyticus
21.5	-	gentiobiose	-	Caldicellulosiruptor saccharolyticus
120	-	lactose	-	Caldicellulosiruptor saccharolyticus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O	Caldicellulosiruptor saccharolyticus	sophorose	beta-D-glucose + D-glucose	-	?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O	Caldicellulosiruptor saccharolyticus	laminaribiose	beta-D-glucose + D-glucose	-	?
4-O-beta-D-galactopyranosyl-D-glucose + H2O	Caldicellulosiruptor saccharolyticus	lactose	beta-D-galactose + D-glucose	-	?
4-O-beta-D-glucopyranosyl-D-glucose + H2O	Caldicellulosiruptor saccharolyticus	cellobiose	beta-D-glucose + D-glucose	-	?
6-O-beta-D-glucopyranosyl-D-glucose + H2O	Caldicellulosiruptor saccharolyticus	gentiobiose	beta-D-glucose + D-glucose	-	?

Organic Solvent Stability

Organic Solvent	Comment	Organism
tetrahydrofuran	unstable above 20%, v/v	Caldicellulosiruptor saccharolyticus

Organism

Organism	UniProt	Comment	Textmining
Caldicellulosiruptor saccharolyticus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
to homogeneity, recombinant enzyme	Caldicellulosiruptor saccharolyticus

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Caldicellulosiruptor saccharolyticus	-
additional information	-	Caldicellulosiruptor saccharolyticus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
560	-	-	Caldicellulosiruptor saccharolyticus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O	salicin	Caldicellulosiruptor saccharolyticus	2-hydroxymethylphenol + beta-D-glucose	-	?
2-nitrophenyl beta-D-galactopyranoside + H2O	-	Caldicellulosiruptor saccharolyticus	2-nitrophenol + beta-D-galactopyranose	-	?
2-nitrophenyl beta-D-glucopyranoside + H2O	-	Caldicellulosiruptor saccharolyticus	2-nitrophenol + beta-D-glucopyranose	-	?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O	sophorose	Caldicellulosiruptor saccharolyticus	beta-D-glucose + D-glucose	-	?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O	laminaribiose	Caldicellulosiruptor saccharolyticus	beta-D-glucose + D-glucose	-	?
4-nitrophenyl beta-D-fucoside + H2O	-	Caldicellulosiruptor saccharolyticus	4-nitrophenol + beta-D-fucose	-	?
4-nitrophenyl beta-D-galactoside + H2O	-	Caldicellulosiruptor saccharolyticus	4-nitrophenol + beta-D-galactose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Caldicellulosiruptor saccharolyticus	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Caldicellulosiruptor saccharolyticus	4-nitrophenol + beta-D-xylose	-	?
4-O-beta-D-galactopyranosyl-D-glucose + H2O	lactose	Caldicellulosiruptor saccharolyticus	beta-D-galactose + D-glucose	-	?
4-O-beta-D-glucopyranosyl-D-glucose + H2O	cellobiose	Caldicellulosiruptor saccharolyticus	beta-D-glucose + D-glucose	-	?
6-O-beta-D-glucopyranosyl-D-glucose + H2O	gentiobiose	Caldicellulosiruptor saccharolyticus	beta-D-glucose + D-glucose	-	?
cellobiose + H2O	-	Caldicellulosiruptor saccharolyticus	2 beta-D-glucose	-	?
gentiobiose + H2O	-	Caldicellulosiruptor saccharolyticus	2 beta-D-glucose	-	?
laminaribiose + H2O	-	Caldicellulosiruptor saccharolyticus	2 beta-D-glucose	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	half-life: 15 h	Caldicellulosiruptor saccharolyticus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	6.5	-	Caldicellulosiruptor saccharolyticus

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Release 2023.1 (January 2023)