Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | stimulation at 10 mM | Thermoclostridium stercorarium |
General Stability | Organism |
---|---|
stabilizing effect by divalent cations | Thermoclostridium stercorarium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | strong inhibition at 1 mM | Thermoclostridium stercorarium | |
p-hydroxymercuribenzoate | strong inhibition at 1 mM | Thermoclostridium stercorarium | |
SDS | complete inhibition at 0.05%, w/v | Thermoclostridium stercorarium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermoclostridium stercorarium | |
33 | - |
cellobiose | - |
Thermoclostridium stercorarium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermoclostridium stercorarium | - |
- |
periplasm | - |
Thermoclostridium stercorarium | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation at 10 mM | Thermoclostridium stercorarium | |
Mg2+ | stimulation at 10 mM | Thermoclostridium stercorarium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
1 * 85000, SDS-PAGE | Thermoclostridium stercorarium |
85000 | 105000 | gel filtration, SDS-PAGE | Thermoclostridium stercorarium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | Thermoclostridium stercorarium | laminaribiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermoclostridium stercorarium | cellobiose | beta-D-glucose + D-glucose | - |
? | |
cellulose + H2O | Thermoclostridium stercorarium | cellulose MN 300 | beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoclostridium stercorarium | - |
- |
- |
Oxidation Stability | Organism |
---|---|
addition of DTT increases thermostability | Thermoclostridium stercorarium |
Purification (Comment) | Organism |
---|---|
to homogeneity, extracellular form, 2step chromatography | Thermoclostridium stercorarium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | two forms, one membrane-bound, one extracellular | Thermoclostridium stercorarium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
31.6 | - |
extracellular beta-glucosidase | Thermoclostridium stercorarium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Thermoclostridium stercorarium | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O | arbutin | Thermoclostridium stercorarium | 4-hydroxyphenol + beta-D-glucopyranose | - |
? | |
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O | laminaribiose | Thermoclostridium stercorarium | beta-D-glucose + D-glucose | - |
? | |
4-methylumbelliferyl-beta-D-glucoside + H2O | - |
Thermoclostridium stercorarium | 4-methylumbelliferone + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | quite specific for beta-1,4-linkages | Thermoclostridium stercorarium | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Thermoclostridium stercorarium | 4-nitrophenol + beta-D-xylose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermoclostridium stercorarium | beta-D-glucose + D-glucose | - |
? | |
Avicel + H2O | acid-swollen, low activity | Thermoclostridium stercorarium | beta-D-glucose + ? | - |
? | |
cellobiose + H2O | - |
Thermoclostridium stercorarium | 2 beta-D-glucose | - |
? | |
cellulose + H2O | cellulose MN 300 | Thermoclostridium stercorarium | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 85000, SDS-PAGE | Thermoclostridium stercorarium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermoclostridium stercorarium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
sharp decline of activity above, increase thermostability by addition of DTT and/or divalent cations | Thermoclostridium stercorarium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Thermoclostridium stercorarium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | optimal activity within | Thermoclostridium stercorarium |