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Literature summary for 3.2.1.21 extracted from

  • Bronnenmeier, K.; Staudenbauer, W.L.
    Purification and properties of an extracellular beta-glucosidase from the cellulolytic thermophile Clostridium stercorarium (1988), Appl. Microbiol. Biotechnol., 28, 380-386.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol stimulation at 10 mM Thermoclostridium stercorarium

General Stability

General Stability Organism
stabilizing effect by divalent cations Thermoclostridium stercorarium

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ strong inhibition at 1 mM Thermoclostridium stercorarium
p-hydroxymercuribenzoate strong inhibition at 1 mM Thermoclostridium stercorarium
SDS complete inhibition at 0.05%, w/v Thermoclostridium stercorarium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
4-nitrophenyl-beta-D-glucopyranoside
-
Thermoclostridium stercorarium
33
-
cellobiose
-
Thermoclostridium stercorarium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermoclostridium stercorarium
-
-
periplasm
-
Thermoclostridium stercorarium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation at 10 mM Thermoclostridium stercorarium
Mg2+ stimulation at 10 mM Thermoclostridium stercorarium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
1 * 85000, SDS-PAGE Thermoclostridium stercorarium
85000 105000 gel filtration, SDS-PAGE Thermoclostridium stercorarium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O Thermoclostridium stercorarium laminaribiose beta-D-glucose + D-glucose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O Thermoclostridium stercorarium cellobiose beta-D-glucose + D-glucose
-
?
cellulose + H2O Thermoclostridium stercorarium cellulose MN 300 beta-D-glucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Thermoclostridium stercorarium
-
-
-

Oxidation Stability

Oxidation Stability Organism
addition of DTT increases thermostability Thermoclostridium stercorarium

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, extracellular form, 2step chromatography Thermoclostridium stercorarium

Source Tissue

Source Tissue Comment Organism Textmining
cell culture two forms, one membrane-bound, one extracellular Thermoclostridium stercorarium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
31.6
-
extracellular beta-glucosidase Thermoclostridium stercorarium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O salicin Thermoclostridium stercorarium 2-hydroxymethylphenol + beta-D-glucose
-
?
(4-hydroxyphenyl)-beta-D-glucopyranoside + H2O arbutin Thermoclostridium stercorarium 4-hydroxyphenol + beta-D-glucopyranose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O laminaribiose Thermoclostridium stercorarium beta-D-glucose + D-glucose
-
?
4-methylumbelliferyl-beta-D-glucoside + H2O
-
Thermoclostridium stercorarium 4-methylumbelliferone + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O quite specific for beta-1,4-linkages Thermoclostridium stercorarium 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O low activity Thermoclostridium stercorarium 4-nitrophenol + beta-D-xylose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose Thermoclostridium stercorarium beta-D-glucose + D-glucose
-
?
Avicel + H2O acid-swollen, low activity Thermoclostridium stercorarium beta-D-glucose + ?
-
?
cellobiose + H2O
-
Thermoclostridium stercorarium 2 beta-D-glucose
-
?
cellulose + H2O cellulose MN 300 Thermoclostridium stercorarium beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 85000, SDS-PAGE Thermoclostridium stercorarium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermoclostridium stercorarium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
sharp decline of activity above, increase thermostability by addition of DTT and/or divalent cations Thermoclostridium stercorarium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Thermoclostridium stercorarium

pH Range

pH Minimum pH Maximum Comment Organism
5 7 optimal activity within Thermoclostridium stercorarium