Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Cellulomonas fimi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Cellulomonas fimi | cellobiose | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | Cellulomonas fimi | only one of two cloned genes is active on cellobiose | beta-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas fimi | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | chomosomal DNA, one gene coding for a cellobiose and aryl-glucoside hydrolysing enzyme, one gene coding for an only aryl-glucoside hydrolyzing enzyme | Cellulomonas fimi | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.21 | - |
- |
Cellulomonas fimi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | one of two cloned genes are only active on this substrate | Cellulomonas fimi | 4-nitrophenol + D-glucopyranose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Cellulomonas fimi | beta-D-glucose + D-glucose | - |
? | |
4-O-beta-D-glucopyranosyl-D-glucose + H2O | only one of two cloned genes is active on cellobiose | Cellulomonas fimi | beta-D-glucose + D-glucose | - |
? |