Literature summary for 3.2.1.21 extracted from

Feldwisch, J.; Vente, A.; Zettl, R.; Bako, L.; Campos, N.; Palme, K.
Characterization of two membrane-associated beta-glucosidases from maize (Zea mays L.) coleoptiles (1994), Biochem. J., 302, 15-21.

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Inhibitors

Inhibitors	Comment	Organism	Structure
1-naphthylacetic acid	competitive inhibition, Ki: 0.43 mM	Zea mays
beta-D-glucose	competitive inhibition, Ki: 148 mM	Zea mays
D-glucono-1,5-lactone	competitive inhibition, Ki: 0.84 mM	Zea mays
indole-3-acetic acid	competitive inhibition, Ki: 1.14 mM	Zea mays

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.65	-	4-nitrophenyl-beta-D-glucopyranoside	-	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Zea mays	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	SDS-PAGE	Zea mays

Organism

Organism	UniProt	Comment	Textmining
Zea mays	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial	Zea mays

Source Tissue

Source Tissue	Comment	Organism	Textmining
coleoptile	-	Zea mays	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
5.4	-	-	Zea mays

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O	low activity	Zea mays	4-nitrophenol + alpha-L-arabinofuranose	-	?
4-nitrophenyl beta-D-galactoside + H2O	low activity	Zea mays	4-nitrophenol + beta-D-galactose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	relatively specific for aryl-beta-D-linked glucopyranoside substrates	Zea mays	4-nitrophenol + D-glucopyranose	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Zea mays

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Release 2023.1 (January 2023)