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Literature summary for 3.2.1.21 extracted from
- Production, purification, and characterization of a highly glucose-tolerant novel beta-glucosidase from Candida peltata (1996), Appl. Environ. Microbiol., 62, 3165-3170.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ethanol | 11% stimulation at 0.75%, v/v | Nakazawaea peltata |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-glucose | highly tolerant to glucose inhibition, Ki: 1.4 M | Nakazawaea peltata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Nakazawaea peltata | |
| 18 | - |
cellohexaose | - |
Nakazawaea peltata | |
| 21 | - |
cellopentaose | - |
Nakazawaea peltata | |
| 35 | - |
cellotetraose | - |
Nakazawaea peltata | |
| 39 | - |
cellotriose | - |
Nakazawaea peltata | |
| 66 | - |
cellobiose | - |
Nakazawaea peltata | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | best enzyme synthesis if cells grown on glucose | Nakazawaea peltata | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Nakazawaea peltata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Nakazawaea peltata | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| cellohexaose + 5 H2O | Nakazawaea peltata | - |
6 D-glucose | - |
? | |
| cellopentaose + 4 H2O | Nakazawaea peltata | - |
5 D-glucose | - |
? | |
| cellotetraose + 3 H2O | Nakazawaea peltata | - |
4 D-glucose | - |
? | |
| cellotriose + 2 H2O | Nakazawaea peltata | - |
3 D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nakazawaea peltata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Nakazawaea peltata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Nakazawaea peltata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108 | - |
- |
Nakazawaea peltata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Nakazawaea peltata | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Nakazawaea peltata | beta-D-glucose + D-glucose | - |
? | |
| cellobiose + H2O | - |
Nakazawaea peltata | 2 beta-D-glucose | - |
? | |
| cellohexaose + 5 H2O | - |
Nakazawaea peltata | 6 D-glucose | - |
? | |
| cellopentaose + 4 H2O | - |
Nakazawaea peltata | 5 D-glucose | - |
? | |
| cellotetraose + 3 H2O | - |
Nakazawaea peltata | 4 D-glucose | - |
? | |
| cellotriose + 2 H2O | - |
Nakazawaea peltata | 3 D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Nakazawaea peltata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Nakazawaea peltata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Nakazawaea peltata |
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