Any feedback?
Literature summary for 3.2.1.21 extracted from
- Production and properties of the linamarase and amygdalase activities of Penicillium aurantiogriseum P35 (1999), Biosci. Biotechnol. Biochem., 63, 805-812.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | strong activation of both PGI and PGII at 10 mM | Penicillium aurantiogriseum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | strong inhibition at 1 mM for PGI and PGII | Penicillium aurantiogriseum | |
| Cu2+ | stronger inhibition for linamarin than for amygdalin at 1 mM, both PGI and PGII | Penicillium aurantiogriseum | |
| D-glucono-1,5-lactone | complete inhibition at 1 mM for PGI and PGII | Penicillium aurantiogriseum | |
| Fe3+ | strong inhibition at 10 mM for PGI and PGII | Penicillium aurantiogriseum | |
| Mn2+ | moderate inhibition at 10 mM for PGI and PGII | Penicillium aurantiogriseum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
amygdalin | for PGI | Penicillium aurantiogriseum | |
| 0.47 | - |
amygdalin | for PGII | Penicillium aurantiogriseum | |
| 2.32 | - |
linamarin | for PGII | Penicillium aurantiogriseum | |
| 2.41 | - |
linamarin | for PGI | Penicillium aurantiogriseum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | most enzyme synthesis if grown in medium containing yeast extract | Penicillium aurantiogriseum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation for PGI at 10 mM | Penicillium aurantiogriseum | |
| Zn2+ | activation at 10 mM for both PGI and PGII | Penicillium aurantiogriseum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61100 | - |
4 * 61100, SDS-PAGE, for PGI | Penicillium aurantiogriseum |
| 71000 | - |
2 * 71000, SDS-PAGE, for PGII | Penicillium aurantiogriseum |
| 147000 | - |
for PGII, gel filtration | Penicillium aurantiogriseum |
| 247000 | - |
for PGI, gel filtration | Penicillium aurantiogriseum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium aurantiogriseum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, preparative isoelectric focusing, 1step chromatography | Penicillium aurantiogriseum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | 2 isoenzymes, PGI and PGII | Penicillium aurantiogriseum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.16 | - |
for PGI | Penicillium aurantiogriseum |
| 0.41 | - |
for PGII | Penicillium aurantiogriseum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O | amygdalin | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| cardiospermin sulfate + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| dhurrin + H2O | low activity for PGI | Penicillium aurantiogriseum | HCN + beta-D-glucose + 4-hydroxybenzaldehyde | - |
? | |
| epivolkenin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| gynocardin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| linamarin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| lucumin + H2O | low activity for PGI, very low activity for PGII | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| passibiflorin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| prunasin + H2O | - |
Penicillium aurantiogriseum | D-mandelonitrile + beta-D-glucose | - |
? | |
| samibunigrin + H2O | best substrate for PGI and PGII | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| taxiphyllin + H2O | low activity for PGI | Penicillium aurantiogriseum | HCN + ? | - |
? | |
| tetraphyllin + H2O | - |
Penicillium aurantiogriseum | HCN + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 71000, SDS-PAGE, for PGII | Penicillium aurantiogriseum |
| tetramer | 4 * 61100, SDS-PAGE, for PGI | Penicillium aurantiogriseum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| linamarase | - |
Penicillium aurantiogriseum |
| PGI | - |
Penicillium aurantiogriseum |
| PGII | - |
Penicillium aurantiogriseum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
for PGI | Penicillium aurantiogriseum |
| 60 | - |
for PGII | Penicillium aurantiogriseum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
for PGI, stable up to | Penicillium aurantiogriseum |
| 60 | - |
for PGII, stable up to | Penicillium aurantiogriseum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
for both PGI and PGII | Penicillium aurantiogriseum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7.5 | for PGI | Penicillium aurantiogriseum |
| 5.5 | 7.5 | for PGII | Penicillium aurantiogriseum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
