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Literature summary for 3.2.1.21 extracted from
- Purification and characterization of beta-D-glucosidase (beta-D-fucosidase) from Bifidobacterium berve clb acclimated to cellobiose (1996), Biosci. Biotechnol. Biochem., 60, 188-193.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
4-nitrophenyl-beta-D-fucoside | - |
Bifidobacterium breve |  |
| 1.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Bifidobacterium breve | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | increasing activity by acclimation with cellobiose | Bifidobacterium breve | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | 48000 | native gradient PAGE | Bifidobacterium breve |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium breve | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Bifidobacterium breve |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Bifidobacterium breve | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-fucoside + H2O | transferase activity for the beta-D-fucosyl group, not for a beta-D-glucosyl group, fucosidase activity specific for intestinal flora | Bifidobacterium breve | 4-nitrophenol + beta-D-fucose | - |
r | |
| 4-nitrophenyl beta-D-galactoside + H2O | low activity | Bifidobacterium breve | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bifidobacterium breve | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Bifidobacterium breve | 4-nitrophenol + beta-D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000-48000, SDS-PAGE, native gradient PAGE | Bifidobacterium breve |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Bifidobacterium breve |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Bifidobacterium breve |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Bifidobacterium breve |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | stable within, kept at 4°C | Bifidobacterium breve |
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