Application | Comment | Organism |
---|---|---|
synthesis | the alpha-transglucosidase-producing Geobacillus stearothermophilus as a potential application technique can be successfully used to prepare industrial isomaltooligosaccharides (IMOs) | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2. 5 l and 20 l scale fermentations are performed. After isolation and purification, liquid alpha-glucosidase preparations are obtained. Through covalent cross-linking and adsorption cross-linking using magnetic chitosan microspheres as the carrier and glutaraldehyde as the crosslinking agent, the conditions for immobilization of alpha-glucosidase on chitosan are determined. Isomaltooligosaccharides (IMOs) are prepared using chitosan membrane-immobilized alpha-glucosidase, beta-amylase, pullulanase, fungal alpha-amylase and starch as substrate. The mixed syrup that contains IMOs is evaluated and analyzed by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC). In addition, small-scale preparation of IMOs is performed. Method, overview | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight inhibition | Geobacillus stearothermophilus | |
Cu2+ | strong inhibition | Geobacillus stearothermophilus | |
Fe2+ | moderate inhibition | Geobacillus stearothermophilus | |
Zn2+ | moderate inhibition | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Geobacillus stearothermophilus | |
K+ | activates | Geobacillus stearothermophilus | |
Mg2+ | activates | Geobacillus stearothermophilus | |
Mn2+ | activates | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | Geobacillus stearothermophilus | - |
alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | Geobacillus stearothermophilus U2 | - |
alpha-D-glucose + D-glucose | - |
? | |
additional information | Geobacillus stearothermophilus | biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 | ? | - |
? | |
additional information | Geobacillus stearothermophilus U2 | biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 | ? | - |
? | |
starch + H2O | Geobacillus stearothermophilus | - |
? | - |
? | |
starch + H2O | Geobacillus stearothermophilus | - |
alpha-D-glucose + ? | - |
? | |
starch + H2O | Geobacillus stearothermophilus U2 | - |
alpha-D-glucose + ? | - |
? | |
starch + H2O | Geobacillus stearothermophilus U2 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus | Q9R5L9 | i.e. Bacillus stearothermophilus | - |
Geobacillus stearothermophilus U2 | - |
- |
- |
Geobacillus stearothermophilus U2 | Q9R5L9 | i.e. Bacillus stearothermophilus | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimization of culture conditions for wild-type and engineered strains, overview | Geobacillus stearothermophilus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus U2 | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucoside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucoside + H2O | - |
Geobacillus stearothermophilus U2 | 4-nitrophenol + alpha-D-glucose | - |
? | |
maltose + H2O | - |
Geobacillus stearothermophilus | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Geobacillus stearothermophilus U2 | alpha-D-glucose + D-glucose | - |
? | |
additional information | biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 | Geobacillus stearothermophilus | ? | - |
? | |
additional information | biotransformation of starch using the alpha-transglucosidase-producing Geobacillus stearothermophilus U2 | Geobacillus stearothermophilus U2 | ? | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus U2 | ? | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus | alpha-D-glucose + ? | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus U2 | alpha-D-glucose + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-transglucosidase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
free enzyme | Geobacillus stearothermophilus |
50 | - |
enzyme immobilized on magnetic chitosan microspheres | Geobacillus stearothermophilus |
60 | - |
- |
Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | the free enzyme is active between 35 and 55°C | Geobacillus stearothermophilus |
40 | 60 | the enzyme immobilized on magnetic chitosan microspheres shows about 40% activity at 30°C, about 50% activity at 40°C, 100% activity at 50°C, and about 90% activity at 60°C | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | the enzyme is thermostable for 24 h and no loss in activity is found below 37°C, but it is inactivated at 65°C or more and completely loses its activity at 80°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.4 | the enzyme is active between pH 6.2 and 7.4 | Geobacillus stearothermophilus |