Application | Comment | Organism |
---|---|---|
synthesis | immobilization of enzyme within agar-agar support via entrapment. The maximum immobilization efficiency of 82.77% is achieved using 4.0% agar-agar keeping the diameter of beads up to 3.0 mm. Km value of immobilized enzyme increases from 1.717 to 2.117 mM whereas Vmax decreases from 8,411 to 7,450 U/min as compared to free enzyme. The immobilization significantly increases the stability of maltase against various temperatures and immobilized maltase retains 100% of its original activity after 2 h at 50°C, whereas the free maltase only shows 60% residual activity under the same conditions. Entrapped maltase can be reused for up to 12 cycles and retains 50% of activity even after the 5th cycle. Agar entrapped maltase retains 73% of its initial activity even after 2 months when stored at 30°C | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.72 | - |
maltose | free enzyme, pH not specified in the publication, temperature not specified in the publication | Bacillus licheniformis | |
2.12 | - |
maltose | immobilized enzyme entrapped in agar, pH not specified in the publication, temperature not specified in the publication | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis KIBGE-IB4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | - |
Bacillus licheniformis | 2 D-glucose | - |
? | |
maltose + H2O | - |
Bacillus licheniformis KIBGE-IB4 | 2 D-glucose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
immobilized enzyme entrapped in agar | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
immobilized maltase retains 100% of its original activity after 2 h at 50°C, whereas the free maltase shows 60% residual activity | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
immobilized enzyme entrapped in agar | Bacillus licheniformis |