Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ferroplasma acidiphilum |
Protein Variants | Comment | Organism |
---|---|---|
H243A | kcat/Km is 23% lower than wild-type value, the iron content is about 55% compared to wild-type enzyme | Ferroplasma acidiphilum |
H275A | kcat/Km is slighly higher than wild-type value, the iron content is similar to that of the wild-type enzyme | Ferroplasma acidiphilum |
H277A | kcat/Km is slighly lower than wild-type value, the iron content is similar to that of the wild-type enzyme | Ferroplasma acidiphilum |
H325A | kcat/Km is slighly higher than wild-type value, the iron content is similar to that of the wild-type enzyme | Ferroplasma acidiphilum |
H390A | kcat/Km is 98% lower than wild-type value, contains no iron | Ferroplasma acidiphilum |
H41A | kcat/Km is slighly higher than wild-type value, the iron content is similar to that of the wild-type enzyme | Ferroplasma acidiphilum |
H47A | kcat/Km is 25% lower than wild-type value, the iron content is about 85% compared to wild-type enzyme | Ferroplasma acidiphilum |
H9A | kcat/Km is slighly higher than wild-type value, the iron content is similar to that of the wild-type enzyme | Ferroplasma acidiphilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-alpha-D-glucopyranosyl fluoride | strong inhibition by the reversible inhibitor, which inhibits immediately upon addition and maintains the same level of inhibition over time | Ferroplasma acidiphilum | |
diethyl dicarbonate | - |
Ferroplasma acidiphilum | |
additional information | no inactivation with 1-ethyl-3-[3-(dimethylamino)propyl]carbodi-imide, tetranitromethane, N-ethylmaleimide, iodoacetate, p-chloromercuribenzoate, 4-chloromercuriphenylsulfonic acid, di-isopropyl fluorophosphate, PMSF. The presence of glucose and maltose, the end-products of the hydrolysis of sucrose, maltose and maltotriose, does not affect the hydrolytic activity of the enzyme | Ferroplasma acidiphilum | |
tosylphenylalanylchloromethane | - |
Ferroplasma acidiphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
sucrose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
0.64 | - |
maltose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
0.69 | - |
maltotriose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
5 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
9.3 | - |
maltotetraose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains 1.04 mol of iron per mol of enzyme | Ferroplasma acidiphilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Ferroplasma acidiphilum |
57300 | - |
x * 57300, calculated from sequence | Ferroplasma acidiphilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ferroplasma acidiphilum | Q5K3Q3 | - |
- |
Ferroplasma acidiphilum DSM 12658 | Q5K3Q3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + alpha-D-glucose | - |
? | |
maltose + H2O | - |
Ferroplasma acidiphilum | 2 D-glucose | - |
? | |
maltose + H2O | - |
Ferroplasma acidiphilum DSM 12658 | 2 D-glucose | - |
? | |
maltotetraose + H2O | very low activity | Ferroplasma acidiphilum | ? | - |
? | |
maltotetraose + H2O | very low activity | Ferroplasma acidiphilum DSM 12658 | ? | - |
? | |
maltotriose + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
maltotriose + H2O | - |
Ferroplasma acidiphilum DSM 12658 | ? | - |
? | |
additional information | the enzyme also shows high transglycosylation activity, it transfers glucosyl groups from maltose to produce maltotriose selectively | Ferroplasma acidiphilum | ? | - |
? | |
additional information | the enzyme also shows high transglycosylation activity, it transfers glucosyl groups from maltose to produce maltotriose selectively | Ferroplasma acidiphilum DSM 12658 | ? | - |
? | |
sucrose + H2O | - |
Ferroplasma acidiphilum | D-glucose + D-fructose | - |
? | |
sucrose + H2O | - |
Ferroplasma acidiphilum DSM 12658 | D-glucose + D-fructose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Ferroplasma acidiphilum |
? | x * 57300, calculated from sequence | Ferroplasma acidiphilum |
Synonyms | Comment | Organism |
---|---|---|
alphaGluFa | - |
Ferroplasma acidiphilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Ferroplasma acidiphilum |
55 | 60 | hydrolysis of sucrose | Ferroplasma acidiphilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 2.5, t1/2: 34 min | Ferroplasma acidiphilum |
55 | - |
thermostability: 57 min | Ferroplasma acidiphilum |
60 | - |
thermostability: 32 vmin | Ferroplasma acidiphilum |
65 | - |
thermostability: less than 5 min | Ferroplasma acidiphilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
maltotetraose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
13 | - |
maltotriose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
22.5 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
94 | - |
sucrose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
126 | - |
maltose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.4 | 3.5 | hydrolysis of sucrose | Ferroplasma acidiphilum |
3 | - |
assay at | Ferroplasma acidiphilum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.5 | 6 | pH 1.5: 74% of maximal activity, pH 2.0: 92% of maximal activity, pH 6.0: about 35% of maximal activity. No measurable activity is detected at pH values above 7.0 and below 1.0 | Ferroplasma acidiphilum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
50°C, t1/2: 34 min | Ferroplasma acidiphilum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0029 | - |
diethyl dicarbonate | pH 3.0, 50°C | Ferroplasma acidiphilum | |
0.0042 | - |
tosylphenylalanylchloromethane | pH 3.0, 50°C | Ferroplasma acidiphilum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ferroplasma acidiphilum | calculated from sequence | - |
6.42 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
maltotetraose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
4.5 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
5.4 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H390A | Ferroplasma acidiphilum | |
18.8 | - |
maltotriose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
197 | - |
maltose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
220 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H47A | Ferroplasma acidiphilum | |
227 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H243A | Ferroplasma acidiphilum | |
291 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H277A | Ferroplasma acidiphilum | |
293 | - |
sucrose | pH 3.0, 50°C, wild-type enzyme | Ferroplasma acidiphilum | |
296 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H325A | Ferroplasma acidiphilum | |
297 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H275A | Ferroplasma acidiphilum | |
302 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H9A | Ferroplasma acidiphilum | |
312 | - |
sucrose | pH 3.0, 50°C, mutant enzyme H41A | Ferroplasma acidiphilum |