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Literature summary for 3.2.1.20 extracted from

  • Motabar, O.; Shi, Z.D.; Goldin, E.; Liu, K.; Southall, N.; Sidransky, E.; Austin, C.P.; Griffiths, G.L.; Zheng, W.
    A new resorufin-based alpha-glucosidase assay for high-throughput screening (2009), Anal. Biochem., 390, 79-84.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.20

Application

Application Comment Organism
medicine therapeutic target for type II diabetes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-butyldeoxynojirimycin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 4-Methylumbelliferyl-alpha-D-glucopyranoside
-
 Homo sapiens
0.166
-
 resorufin alpha-D-glucopyranoside
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-D-glucopyranoside + H2O assay at room temperature, 10 min incubation, 7.4 nM enzyme concentration Homo sapiens 4-methylumbelliferone + alpha-D-glucose
-
 ?
resorufin alpha-D-glucopyranoside + H2O assay at room temperature, 10 min incubation, 7.4 nM enzyme concentration Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-glucosidase
-
 Homo sapiens
GAA
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0068
-
 resorufin alpha-D-glucopyranoside as substrate Homo sapiens N-butyldeoxynojirimycin
0.0092
-
 4-methylumbelliferyl-alpha-D-glucopyranoside as substrate Homo sapiens N-butyldeoxynojirimycin

General Information

General Information Comment Organism
malfunction enzyme mutations cause accumulation of glycogen in lysosomes, resulting in Pompe disease Homo sapiens