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Literature summary for 3.2.1.20 extracted from

  • Giannesi, G.C.; Polizeli, M.de L.; Terenzi, H.F.; Jorge, J.A.
    A novel alpha-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme (2006), Process Biochem., 41, 1729-1735.
No PubMed abstract available
Search for more literature for 3.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 1 mM Thermochaetoides thermophila
Al3+ 35% inhibition at 1 mM Thermochaetoides thermophila
Co2+ 51% inhibition at 1 mM Thermochaetoides thermophila
Cu2+ 46% inhibition at 1 mM Thermochaetoides thermophila
Hg2+ complete inhibition at 1 mM Thermochaetoides thermophila
trehalose inhibits both glycosyl hydrolase and transglycosylase activities decreasing Vmax by 3.9-4.8fold Thermochaetoides thermophila
Zn2+ 70% inhibition at 1 mM Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4.35 mg/ml for starch Thermochaetoides thermophila
0.55
-
 maltose pH 7.0, 60°C Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not affected by 1 mM of Mg2+, Ca2+, Ba2+, K+, Fe2+, NH4+, Mn2+, EDTA, and 2-mercaptoethanol Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila
26000
-
 2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila
107000
-
 gel filtration Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
 var. coprophilum, filamentous fungus, strain ATCC 1645
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 14% carbohydrate content Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
native enzyme 64fold by anion exchange chromatography and two steps of hydrophobic interaction chromatography, to homogeneity Thermochaetoides thermophila

Source Tissue

Source Tissue Comment Organism Textmining
additional information highest enzyme activity in the cells after growth on maltose or starch, effects of sugars as carbon sources on enzyme activity, overview Thermochaetoides thermophila
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.33
-
 purified enzyme Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + H2O high activity Thermochaetoides thermophila alpha-D-glucose
-
 ?
isomaltose + H2O low activity Thermochaetoides thermophila 2 alpha-D-glucose
-
 ?
maltooligosaccharides + H2O
-
 Thermochaetoides thermophila alpha-D-glucose
-
 ?
maltose + H2O preferred substrate Thermochaetoides thermophila alpha-D-glucose + D-glucose
-
 ?
additional information substrate specificity, overview, no activity with trehalose, the enzyme exhibits transglycosylation activity producing trehalose and maltooligosaccharides from 5% maltose, at early stages of the transglycosylation reaction trehalose is the predominant product, while at later stages maltooligosaccharides are also accumulated Thermochaetoides thermophila ?
-
 ?
starch + H2O
-
 Thermochaetoides thermophila alpha-D-glucose
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 70 10% of maximal activity at 35°C, 33% at 70°C, steady increase in activity from 35 to 60°C, sharp drop of activity above 65°C Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermochaetoides thermophila

pH Range

pH Minimum pH Maximum Comment Organism
4 7.5 over 50% of maximal activity within this range, inactive at pH 4.0 and pH 8.0 Thermochaetoides thermophila