Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain JM109 | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
A200V | site-directed mutagenesis, the mutant is almost inactive | Geobacillus stearothermophilus |
A200V/S202N/H203M | site-directed mutagenesis, the mutant shows altered substrate specificity and highly reduced activity compared to the wild-type enzyme | Geobacillus stearothermophilus |
N258P | site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme with reduced activity against maltose and increased activity against isomaltose | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltooligosaccharides + H2O | Geobacillus stearothermophilus | - |
alpha-D-glucose | - |
? | |
maltooligosaccharides + H2O | Geobacillus stearothermophilus ATCC 12016 | - |
alpha-D-glucose | - |
? | |
maltose + H2O | Geobacillus stearothermophilus | - |
alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | Geobacillus stearothermophilus ATCC 12016 | - |
alpha-D-glucose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
strain ATCC12016 | - |
Geobacillus stearothermophilus ATCC 12016 | - |
strain ATCC12016 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain JM109 by heat treatment at 55°C for 10 min, followed by anion exchange, adsorption, hydrophobic interaction chromatography, and gel filtration | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
purified recombinant mutant A200V, substrate maltose | Geobacillus stearothermophilus |
3280 | - |
purified recombinant mutant N258P, substrate maltose | Geobacillus stearothermophilus |
6890 | - |
purified recombinant wild-type enzyme, substrate maltose | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + alpha-D-glucose | - |
? | |
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Geobacillus stearothermophilus ATCC 12016 | 4-nitrophenol + alpha-D-glucose | - |
? | |
isomaltose + H2O | - |
Geobacillus stearothermophilus | 2 alpha-D-glucose | - |
? | |
isomaltose + H2O | - |
Geobacillus stearothermophilus ATCC 12016 | 2 alpha-D-glucose | - |
? | |
maltooligosaccharides + H2O | - |
Geobacillus stearothermophilus | alpha-D-glucose | - |
? | |
maltooligosaccharides + H2O | - |
Geobacillus stearothermophilus ATCC 12016 | alpha-D-glucose | - |
? | |
maltose + H2O | - |
Geobacillus stearothermophilus | alpha-D-glucose + D-glucose | - |
? | |
maltose + H2O | - |
Geobacillus stearothermophilus ATCC 12016 | alpha-D-glucose + D-glucose | - |
? | |
additional information | the enzyme specifically hydrolyzes alpha-1,4-glucosidic bonds in maltose, maltooligosaccharides, and alpha-glucans, isomaltose is a poor substrate, residues Ala200 and Asn258 in the conserved region II and III, respectively, are largely responsible for substrate recognition | Geobacillus stearothermophilus | ? | - |
? | |
additional information | the enzyme specifically hydrolyzes alpha-1,4-glucosidic bonds in maltose, maltooligosaccharides, and alpha-glucans, isomaltose is a poor substrate, residues Ala200 and Asn258 in the conserved region II and III, respectively, are largely responsible for substrate recognition | Geobacillus stearothermophilus ATCC 12016 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme contains four conserved regions I-IV | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-glucosidase | - |
Geobacillus stearothermophilus |
More | the enzyme belongs to the glycoside hydrolase family 13 | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7 | assay at | Geobacillus stearothermophilus |