Literature summary for 3.2.1.20 extracted from
Chanchao, C.; Padoongsupalai, R.; Sangvanich, P.
Expression and characterization of alpha-glucosidase III in the dwarf honeybee, Apis florea (Hymenoptera: Apoidea: Apidae) (2007), Insect Sci., 14, 283-293.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
DNA and amino acid sequence determination and anaylsis, semi-quantitative expression analysis, sequence comparison |
Apis florea |
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
73000 |
- |
gel filtration |
Apis florea |
75000 |
- |
1 * 75000, SDS-PAGE |
Apis florea |
Organism
Organism |
UniProt |
Comment |
Textmining |
Apis florea |
A5JVZ2 |
dwarf honeybee |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
native enzyme 13.4fold by ammonium sulfate fractination, anion exchange chromatography, and gel filtration |
Apis florea |
Source Tissue
Source Tissue |
Comment |
Organism |
Textmining |
additional information |
expression levels at different developmental stages, highest expressionlevel in forager bees, no detectable expression in eggs and only a low expression level in nurse bees, overview |
Apis florea |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
additional information |
the enzyme is active with sucrose |
Apis florea |
? |
- |
? |
|
Subunits
Subunits |
Comment |
Organism |
monomer |
1 * 75000, SDS-PAGE |
Apis florea |
Synonyms
Synonyms |
Comment |
Organism |
alpha-glucosidase III |
- |
Apis florea |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
30 |
- |
assay at |
Apis florea |
45 |
- |
in-gel activity assay |
Apis florea |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
5 |
- |
assay at |
Apis florea |