Literature summary for 3.2.1.20 extracted from

Naested, H.; Kramhoft, B.; Lok, F.; Bojsen, K.; Yu, S.; Svensson, B.
Production of enzymatically active recombinant full-length barley high pI alpha-glucosidase of glycoside family 31 by high cell-density fermentation of Pichia pastoris and affinity purification (2006), Protein Expr. Purif., 46, 56-63.

Search for more literature for 3.2.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
gene agl97, expression of functional His-tagged full-length enzyme in Pichia pastoris, production by high cell-density fermentation	Hordeum vulgare

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	maltose	pH 4.5, 37°C, recombinant enzyme	Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
92000	-	wild-type enzyme, gel filtration	Hordeum vulgare
100000	-	recombinant enzyme, gel filtration	Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
maltose + H2O	Hordeum vulgare	-	alpha-D-glucose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Hordeum vulgare	Q9LLY2	gene agl97	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme 25fold from Pichia pastoris by dia- and ultrafiltration, nickel affinity chromatography and gel filtration	Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
25.6	-	purified recombinant enzyme	Hordeum vulgare

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
maltose + H2O	-	Hordeum vulgare	alpha-D-glucose + D-glucose	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 100000, recombinant enzyme, SDS-PAGE	Hordeum vulgare

Synonyms

Synonyms	Comment	Organism
high pI alpha-glucosidase	-	Hordeum vulgare
More	the enzyme belongs to the alpha-glucosidase family 31	Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Hordeum vulgare

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
81.7	-	maltose	pH 4.5, 37°C, recombinant enzyme	Hordeum vulgare

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.5	-	assay at	Hordeum vulgare

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Release 2023.1 (January 2023)