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Literature summary for 3.2.1.20 extracted from
- Purification, characterization, and synergistic action of phytate-resistant alpha-amylase and alpha-glucosidase from Geobacillus thermodenitrificans HRO10 (2006), J. Biotechnol., 125, 27-38.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | complete inhibition at 2 mM | Geobacillus thermodenitrificans |  |
| EDTA | 22% inhibition at 10 mM | Geobacillus thermodenitrificans | |
| iodoacetic acid | 23% inhibition at 10 mM | Geobacillus thermodenitrificans | |
| additional information | no inhibition by phytate | Geobacillus thermodenitrificans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the enzyme exhibits Michaelis-Menten kinetics | Geobacillus thermodenitrificans | |
| 17 | - |
maltose | pH 7.0, 55°C | Geobacillus thermodenitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | Geobacillus thermodenitrificans | - |
alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | Geobacillus thermodenitrificans HRO10 | - |
alpha-D-glucose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus thermodenitrificans | - |
- |
- |
| Geobacillus thermodenitrificans HRO10 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 25.4fold to homogeneity by ultrafiltration, chromatofocusing, and gel filtration | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltose + H2O | - |
Geobacillus thermodenitrificans | alpha-D-glucose + D-glucose | - |
? | |
| maltose + H2O | - |
Geobacillus thermodenitrificans HRO10 | alpha-D-glucose + D-glucose | - |
? | |
| additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans | ? | - |
? | |
| additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans HRO10 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Geobacillus thermodenitrificans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | 30% of maximal activity at 35°C, 80% of maximal activity at 45-60°C, inactive above 75°C | Geobacillus thermodenitrificans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
purified enzyme, 30 min, 70% activity remaining | Geobacillus thermodenitrificans |
| 70 | - |
purified enzyme, 20 min, 3.7% activity remaining | Geobacillus thermodenitrificans |
| 75 | - |
inactivation | Geobacillus thermodenitrificans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Geobacillus thermodenitrificans |
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