Literature summary for 3.2.1.20 extracted from

Ernst, H.A.; Willemoes, M.; Lo Leggio, L.; Leonard, G.; Blum, P.; Larsen, S.
Characterization of different crystal forms of the alpha-glucosidase MalA from Sulfolobus solfataricus (2005), Acta Crystallogr. Sect. F, 61, 1039-1042.

Cloned(Commentary)

Cloned (Comment)	Organism
gene malA, overexpression of wild-type and selenomethionine-labeled enzyme in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 2-5 mg/ml protein in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl, 0.002 ml protein solution is mixed with 0.003 ml reservoir solution and equilibrated against 1 ml reservoir solution, different compositions of the reservoir solution result in different crystal forms, overview, X-ray diffraction structure determination and analysis of the different crystal forms at 2.5-4.2 A resolution	Saccharolobus solfataricus

Crystallization (Comment)

Organism

purified recombinant wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 2-5 mg/ml protein in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl, 0.002 ml protein solution is mixed with 0.003 ml reservoir solution and equilibrated against 1 ml reservoir solution, different compositions of the reservoir solution result in different crystal forms, overview, X-ray diffraction structure determination and analysis of the different crystal forms at 2.5-4.2 A resolution

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	gene malA	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and selenomethionine-labeled enzyme from Escherichia coli by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
MalA	-	Saccharolobus solfataricus
More	the enzyme belongs to the alpha-glucosidase family 31	Saccharolobus solfataricus

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Release 2023.1 (January 2023)