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Literature summary for 3.2.1.20 extracted from

  • Shanmugam, V.; Sriram, S.; Babu, S.; Nandakumar, R.; Raguchander, T.; Balasubramanian, P.; Samiyappan, R.
    Purification and characterization of an extracellular alpha-glucosidase protein from Trichoderma viride which degrades a phytotoxin associated with sheath blight disease in rice (2001), J. Appl. Microbiol., 90, 320-329.
    View publication on PubMed
Search for more literature for 3.2.1.20

Application

Application Comment Organism
biotechnology expression of the enzyme in rice plants can transduce enhanced resistance against sheath blight pathogen Rhizoctonia solani by inactivation of the RS toxin Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.2, 55°C Trichoderma viride

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion Trichoderma viride
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
 native PAGE Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
RS toxin + H2O Trichoderma viride specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth ?
-
 ?
RS toxin + H2O Trichoderma viride TvMNT7 specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth ?
-
 ?

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-
Trichoderma viride TvMNT7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma viride

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Trichoderma viride 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Trichoderma viride TvMNT7 4-nitrophenol + alpha-D-glucopyranose
-
 ?
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Trichoderma viride 4-nitrophenol + alpha-D-mannopyranose
-
 ?
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
 Trichoderma viride TvMNT7 4-nitrophenol + alpha-D-mannopyranose
-
 ?
maltose + H2O
-
 Trichoderma viride 2 D-glucose
-
 ?
maltose + H2O
-
 Trichoderma viride TvMNT7 2 D-glucose
-
 ?
RS toxin + H2O specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth Trichoderma viride ?
-
 ?
RS toxin + H2O host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin, preferred substrate Trichoderma viride ?
-
 ?
RS toxin + H2O specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth Trichoderma viride TvMNT7 ?
-
 ?
RS toxin + H2O host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin, preferred substrate Trichoderma viride TvMNT7 ?
-
 ?
starch + H2O
-
 Trichoderma viride D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 110000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Trichoderma viride

pI Value

Organism Comment pI Value Maximum pI Value
Trichoderma viride isoelectric focusing
-
 4.9