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Literature summary for 3.2.1.20 extracted from

  • Kobayashi, I.; Tokuda, M.; Hashimoto, H.; Konda, T.; Nakano, H.; Kitahata, S.
    Purification and characterization of a new type of alpha-glucosidase from Paecilomyces lilacinus that has transglucosylation activity to produce alpha-1,3- and alpha-1,2-linked oligosaccharides (2003), Biosci. Biotechnol. Biochem., 67, 29-35.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
nigerose pH 5.0, 50°C Purpureocillium lilacinum
0.16
-
maltose pH 5.0, 50°C Purpureocillium lilacinum
0.86
-
isomaltose pH 5.0, 50°C Purpureocillium lilacinum
1.44
-
kojibiose pH 5.0, 50°C Purpureocillium lilacinum
2.8
-
starch soluble substrate, pH 5.0, 50°C Purpureocillium lilacinum

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Purpureocillium lilacinum 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
x * 54000 Purpureocillium lilacinum

Organism

Organism UniProt Comment Textmining
Purpureocillium lilacinum
-
-
-
Purpureocillium lilacinum HKS-124
-
-
-

Purification (Commentary)

Purification (Comment) Organism
254fold Purpureocillium lilacinum

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Purpureocillium lilacinum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.4
-
purified enzyme Purpureocillium lilacinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylose + H2O
-
Purpureocillium lilacinum D-glucose + ?
-
?
D-glucose + kojibiose
-
Purpureocillium lilacinum 4-O-alpha-kojibiosyl-D-glucose
-
r
D-glucose + maltose transglycosylation activity Purpureocillium lilacinum maltotriose
-
r
D-glucose + nigerose
-
Purpureocillium lilacinum 4-O-alpha-nigerosyl-D-glucose
-
r
glycogen + H2O low activity Purpureocillium lilacinum D-glucose + ?
-
?
isomaltose + H2O
-
Purpureocillium lilacinum 2 alpha-D-glucose
-
?
kojibiose + H2O
-
Purpureocillium lilacinum 2 D-glucose
-
?
kojibiose + H2O
-
Purpureocillium lilacinum HKS-124 2 D-glucose
-
?
kojibiose + maltose transglycosylation activity Purpureocillium lilacinum 4-O-alpha-kojibiosyl-D-glucose + D-glucose
-
?
maltohexaose + H2O
-
Purpureocillium lilacinum maltopentaose + D-glucose
-
?
maltopentaose + H2O
-
Purpureocillium lilacinum D-glucose + ?
-
?
maltose + H2O
-
Purpureocillium lilacinum 2 D-glucose
-
?
maltose + H2O
-
Purpureocillium lilacinum HKS-124 2 D-glucose
-
?
maltotetraose + H2O
-
Purpureocillium lilacinum maltotriose + D-glucose
-
?
maltotriose + H2O
-
Purpureocillium lilacinum maltose + D-glucose
-
?
maltotriose + H2O
-
Purpureocillium lilacinum HKS-124 maltose + D-glucose
-
?
additional information the enzyme has high transglucosylation activity synthesizing oligosaccharides containing alpha-1,3- and alpha-1,2-linkages, overview, 4-nitrophenyl alpha-D-glucopyranoside and isomaltose are poor substrates, no activity with trehalose, sucrose, dextran, cellobiose, pullulan, inullin, and cyclodextrin Purpureocillium lilacinum ?
-
?
additional information the enzyme has high transglucosylation activity synthesizing oligosaccharides containing alpha-1,3- and alpha-1,2-linkages, overview, 4-nitrophenyl alpha-D-glucopyranoside and isomaltose are poor substrates, no activity with trehalose, sucrose, dextran, cellobiose, pullulan, inullin, and cyclodextrin Purpureocillium lilacinum HKS-124 ?
-
?
nigerose + H2O best substrate Purpureocillium lilacinum 2 D-glucose
-
?
nigerose + H2O best substrate Purpureocillium lilacinum HKS-124 2 D-glucose
-
?
nigerose + maltose transglycosylation activity Purpureocillium lilacinum 4-O-alpha-nigerosyl-D-glucose + D-glucose
-
?
starch + H2O soluble starch Purpureocillium lilacinum D-glucose + ?
-
?

Subunits

Subunits Comment Organism
? x * 54000 Purpureocillium lilacinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Purpureocillium lilacinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Purpureocillium lilacinum

pI Value

Organism Comment pI Value Maximum pI Value
Purpureocillium lilacinum isoelectric focusing 4.7 4.6